EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY

Anti-p-azophenyl-β-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. S...

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Detalles Bibliográficos
Autores principales: Rockey, John H., Klinman, Norman R., Karush, Fred
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137781/
https://www.ncbi.nlm.nih.gov/pubmed/14212122
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author Rockey, John H.
Klinman, Norman R.
Karush, Fred
author_facet Rockey, John H.
Klinman, Norman R.
Karush, Fred
author_sort Rockey, John H.
collection PubMed
description Anti-p-azophenyl-β-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S β(2A)-globulin, 19S γ-globulin, a 10S γ(1) globulin, and three antigenically distinct 7S γ-globulins. The specific hapten-binding activity of the 7S β(2A)-antibody and of the 10S γ(1)-antibody has been demonstrated by equilibrium dialysis and by analytical ultracentrifugation using a combination of schlieren and absorption optics. The 10S γ(1)-globulin antibody may be representative of a hitherto undescribed class of immunoglobulins.
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spelling pubmed-21377812008-04-17 EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY Rockey, John H. Klinman, Norman R. Karush, Fred J Exp Med Article Anti-p-azophenyl-β-lactoside (Lac) antibody has been isolated from equine antiserum by specific precipitation with Lac-HSA (human serum albumin) and Lac-Hy (hemocyanin). Antibody was separated from antigen by chromatography on DEAE cellulose and zone electrophoresis in solvents containing lactose. Six antigenically distinct immunoglobulins have been identified in purified equine anti-Lac antibody: 7S β(2A)-globulin, 19S γ-globulin, a 10S γ(1) globulin, and three antigenically distinct 7S γ-globulins. The specific hapten-binding activity of the 7S β(2A)-antibody and of the 10S γ(1)-antibody has been demonstrated by equilibrium dialysis and by analytical ultracentrifugation using a combination of schlieren and absorption optics. The 10S γ(1)-globulin antibody may be representative of a hitherto undescribed class of immunoglobulins. The Rockefeller University Press 1964-10-01 /pmc/articles/PMC2137781/ /pubmed/14212122 Text en Copyright © 1964 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rockey, John H.
Klinman, Norman R.
Karush, Fred
EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY
title EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY
title_full EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY
title_fullStr EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY
title_full_unstemmed EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY
title_short EQUINE ANTIHAPTEN ANTIBODY : I. 7S β(2A)- AND 10S γ(1)-GLOBULIN COMPONENTS OF PURIFIED ANTI-β-LACTOSIDE ANTIBODY
title_sort equine antihapten antibody : i. 7s β(2a)- and 10s γ(1)-globulin components of purified anti-β-lactoside antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137781/
https://www.ncbi.nlm.nih.gov/pubmed/14212122
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