RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN

Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L...

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Detalles Bibliográficos
Autores principales: Metzger, Henry, Mannik, Mart
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137869/
https://www.ncbi.nlm.nih.gov/pubmed/14247718
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author Metzger, Henry
Mannik, Mart
author_facet Metzger, Henry
Mannik, Mart
author_sort Metzger, Henry
collection PubMed
description Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially interacted with the anti-DNP L chains. Antibody H chain-antibody L chain recombinants formed in the presence of hapten were more active than the corresponding recombinants formed in the absence of hapten. Speculations are made regarding the possible mechanisms and biological significance of these effects.
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spelling pubmed-21378692008-04-17 RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN Metzger, Henry Mannik, Mart J Exp Med Article Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially interacted with the anti-DNP L chains. Antibody H chain-antibody L chain recombinants formed in the presence of hapten were more active than the corresponding recombinants formed in the absence of hapten. Speculations are made regarding the possible mechanisms and biological significance of these effects. The Rockefeller University Press 1964-10-31 /pmc/articles/PMC2137869/ /pubmed/14247718 Text en Copyright © 1964 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Metzger, Henry
Mannik, Mart
RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN
title RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN
title_full RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN
title_fullStr RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN
title_full_unstemmed RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN
title_short RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN
title_sort recombination of antibody polypeptide chains in the presence of antigen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137869/
https://www.ncbi.nlm.nih.gov/pubmed/14247718
work_keys_str_mv AT metzgerhenry recombinationofantibodypolypeptidechainsinthepresenceofantigen
AT mannikmart recombinationofantibodypolypeptidechainsinthepresenceofantigen

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