Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen

The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW® RSV ELISA, the NucliSENS® EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compare...

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Detalles Bibliográficos
Autores principales: Tillmann, Ramona Liza, Simon, Arne, Müller, Andreas, Schildgen, Oliver
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137935/
https://www.ncbi.nlm.nih.gov/pubmed/18159240
http://dx.doi.org/10.1371/journal.pone.0001357
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author Tillmann, Ramona Liza
Simon, Arne
Müller, Andreas
Schildgen, Oliver
author_facet Tillmann, Ramona Liza
Simon, Arne
Müller, Andreas
Schildgen, Oliver
author_sort Tillmann, Ramona Liza
collection PubMed
description The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW® RSV ELISA, the NucliSENS® EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.
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spelling pubmed-21379352007-12-26 Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen Tillmann, Ramona Liza Simon, Arne Müller, Andreas Schildgen, Oliver PLoS One Research Article The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW® RSV ELISA, the NucliSENS® EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics. Public Library of Science 2007-12-26 /pmc/articles/PMC2137935/ /pubmed/18159240 http://dx.doi.org/10.1371/journal.pone.0001357 Text en Tillmann et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tillmann, Ramona Liza
Simon, Arne
Müller, Andreas
Schildgen, Oliver
Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
title Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
title_full Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
title_fullStr Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
title_full_unstemmed Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
title_short Sensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
title_sort sensitive commercial nasba assay for the detection of respiratory syncytial virus in clinical specimen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137935/
https://www.ncbi.nlm.nih.gov/pubmed/18159240
http://dx.doi.org/10.1371/journal.pone.0001357
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AT schildgenoliver sensitivecommercialnasbaassayforthedetectionofrespiratorysyncytialvirusinclinicalspecimen

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