Cargando…

Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases

PURPOSE: We have previously demonstrated in a pilot study of 348 invasive breast cancers that mast cell (MC) infiltrates within primary breast cancers are associated with a good prognosis. Our aim was to verify this finding in a larger cohort of invasive breast cancer patients and examine the relati...

Descripción completa

Detalles Bibliográficos
Autores principales: Rajput, Ashish B., Turbin, Dmitry A., Cheang, Maggie CU, Voduc, David K., Leung, Sam, Gelmon, Karen A., Gilks, C. Blake, Huntsman, David G.
Formato: Texto
Lenguaje:English
Publicado: Springer US 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137942/
https://www.ncbi.nlm.nih.gov/pubmed/17431762
http://dx.doi.org/10.1007/s10549-007-9546-3
_version_ 1782143447433478144
author Rajput, Ashish B.
Turbin, Dmitry A.
Cheang, Maggie CU
Voduc, David K.
Leung, Sam
Gelmon, Karen A.
Gilks, C. Blake
Huntsman, David G.
author_facet Rajput, Ashish B.
Turbin, Dmitry A.
Cheang, Maggie CU
Voduc, David K.
Leung, Sam
Gelmon, Karen A.
Gilks, C. Blake
Huntsman, David G.
author_sort Rajput, Ashish B.
collection PubMed
description PURPOSE: We have previously demonstrated in a pilot study of 348 invasive breast cancers that mast cell (MC) infiltrates within primary breast cancers are associated with a good prognosis. Our aim was to verify this finding in a larger cohort of invasive breast cancer patients and examine the relationship between the presence of MCs and other clinical and pathological features. EXPERIMENTAL DESIGN: Clinically annotated tissue microarrays (TMAs) containing 4,444 cases were constructed and stained with c-Kit (CD-117) using standard immunoperoxidase techniques to identify and quantify MCs. For statistical analysis, we applied a split-sample validation technique. Breast cancer specific survival was analyzed by Kaplan–Meier [KM] method and log rank test was used to compare survival curves. RESULTS: Survival analysis by KM method showed that the presence of stromal MCs was a favourable prognostic factor in the training set (P = 0.001), and the validation set group (P = 0.006). X-tile plot generated to define the optimal number of MCs showed that the presence of any number of stromal MCs predicted good prognosis. Multivariate analysis showed that the MC effect in the training set (Hazard ratio [HR] = 0.804, 95% Confidence interval [CI], 0.653–0.991, P = 0.041) and validation set analysis (HR = 0.846, 95% CI, 0.683–1.049, P = 0.128) was independent of age, tumor grade, tumor size, lymph node, ER and Her2 status. CONCLUSIONS: This study concludes that stromal MC infiltration in invasive breast cancer is an independent good prognostic marker and reiterates the critical role of local inflammatory responses in breast cancer progression.
format Text
id pubmed-2137942
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-21379422007-12-14 Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases Rajput, Ashish B. Turbin, Dmitry A. Cheang, Maggie CU Voduc, David K. Leung, Sam Gelmon, Karen A. Gilks, C. Blake Huntsman, David G. Breast Cancer Res Treat Preclinical Study PURPOSE: We have previously demonstrated in a pilot study of 348 invasive breast cancers that mast cell (MC) infiltrates within primary breast cancers are associated with a good prognosis. Our aim was to verify this finding in a larger cohort of invasive breast cancer patients and examine the relationship between the presence of MCs and other clinical and pathological features. EXPERIMENTAL DESIGN: Clinically annotated tissue microarrays (TMAs) containing 4,444 cases were constructed and stained with c-Kit (CD-117) using standard immunoperoxidase techniques to identify and quantify MCs. For statistical analysis, we applied a split-sample validation technique. Breast cancer specific survival was analyzed by Kaplan–Meier [KM] method and log rank test was used to compare survival curves. RESULTS: Survival analysis by KM method showed that the presence of stromal MCs was a favourable prognostic factor in the training set (P = 0.001), and the validation set group (P = 0.006). X-tile plot generated to define the optimal number of MCs showed that the presence of any number of stromal MCs predicted good prognosis. Multivariate analysis showed that the MC effect in the training set (Hazard ratio [HR] = 0.804, 95% Confidence interval [CI], 0.653–0.991, P = 0.041) and validation set analysis (HR = 0.846, 95% CI, 0.683–1.049, P = 0.128) was independent of age, tumor grade, tumor size, lymph node, ER and Her2 status. CONCLUSIONS: This study concludes that stromal MC infiltration in invasive breast cancer is an independent good prognostic marker and reiterates the critical role of local inflammatory responses in breast cancer progression. Springer US 2007-03-13 2008-01 /pmc/articles/PMC2137942/ /pubmed/17431762 http://dx.doi.org/10.1007/s10549-007-9546-3 Text en © Springer Science+Business Media, LLC 2007
spellingShingle Preclinical Study
Rajput, Ashish B.
Turbin, Dmitry A.
Cheang, Maggie CU
Voduc, David K.
Leung, Sam
Gelmon, Karen A.
Gilks, C. Blake
Huntsman, David G.
Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
title Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
title_full Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
title_fullStr Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
title_full_unstemmed Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
title_short Stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
title_sort stromal mast cells in invasive breast cancer are a marker of favourable prognosis: a study of 4,444 cases
topic Preclinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137942/
https://www.ncbi.nlm.nih.gov/pubmed/17431762
http://dx.doi.org/10.1007/s10549-007-9546-3
work_keys_str_mv AT rajputashishb stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT turbindmitrya stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT cheangmaggiecu stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT voducdavidk stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT leungsam stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT gelmonkarena stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT gilkscblake stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases
AT huntsmandavidg stromalmastcellsininvasivebreastcancerareamarkeroffavourableprognosisastudyof4444cases