ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS

Complement-fixing anti-DNA antibodies were detected in a minority of sera of rabbits hyperimmunized with killed Gram-negative bacteria. The C'-fixing property of DNA was lost after DNase treatment. Preferential reactivity with denatured DNA was observed. The antisera reacted with DNA preparatio...

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Detalles Bibliográficos
Autores principales: Christian, Charles L., DeSimone, Arthur R., Abruzzo, John L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137977/
https://www.ncbi.nlm.nih.gov/pubmed/14264274
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author Christian, Charles L.
DeSimone, Arthur R.
Abruzzo, John L.
author_facet Christian, Charles L.
DeSimone, Arthur R.
Abruzzo, John L.
author_sort Christian, Charles L.
collection PubMed
description Complement-fixing anti-DNA antibodies were detected in a minority of sera of rabbits hyperimmunized with killed Gram-negative bacteria. The C'-fixing property of DNA was lost after DNase treatment. Preferential reactivity with denatured DNA was observed. The antisera reacted with DNA preparations derived from rabbit bone marrow and thymus, calf thymus, pneumococci, salmon sperm, and Escherichia coli. E. coli DNA was less effective than preparations of mammalian and salmon sperm DNA in fixation of C'. Inhibition of DNA C' fixation by nucleotides and nucleosides was observed. The bulk of anti-DNA activity was associated with the low molecular weight antibody fraction.
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spelling pubmed-21379772008-04-17 ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS Christian, Charles L. DeSimone, Arthur R. Abruzzo, John L. J Exp Med Article Complement-fixing anti-DNA antibodies were detected in a minority of sera of rabbits hyperimmunized with killed Gram-negative bacteria. The C'-fixing property of DNA was lost after DNase treatment. Preferential reactivity with denatured DNA was observed. The antisera reacted with DNA preparations derived from rabbit bone marrow and thymus, calf thymus, pneumococci, salmon sperm, and Escherichia coli. E. coli DNA was less effective than preparations of mammalian and salmon sperm DNA in fixation of C'. Inhibition of DNA C' fixation by nucleotides and nucleosides was observed. The bulk of anti-DNA activity was associated with the low molecular weight antibody fraction. The Rockefeller University Press 1965-01-31 /pmc/articles/PMC2137977/ /pubmed/14264274 Text en Copyright © 1965 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Christian, Charles L.
DeSimone, Arthur R.
Abruzzo, John L.
ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS
title ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS
title_full ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS
title_fullStr ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS
title_full_unstemmed ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS
title_short ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS
title_sort anti-dna antibodies in hyperimmunized rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137977/
https://www.ncbi.nlm.nih.gov/pubmed/14264274
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AT desimonearthurr antidnaantibodiesinhyperimmunizedrabbits
AT abruzzojohnl antidnaantibodiesinhyperimmunizedrabbits

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