QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD

A technique for in vivo quantitation of transplantation immunity has been presented. Known numbers of H(3)-thymidine-labeled lymphoid cells were injected intravenously into isologous or homologous recipients. The total radioactivity in host spleens was counted at different time intervals. The rate o...

Descripción completa

Detalles Bibliográficos
Autores principales: Najarian, John S., Feldman, Joseph D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137990/
https://www.ncbi.nlm.nih.gov/pubmed/14276774
_version_ 1782143458687844352
author Najarian, John S.
Feldman, Joseph D.
author_facet Najarian, John S.
Feldman, Joseph D.
author_sort Najarian, John S.
collection PubMed
description A technique for in vivo quantitation of transplantation immunity has been presented. Known numbers of H(3)-thymidine-labeled lymphoid cells were injected intravenously into isologous or homologous recipients. The total radioactivity in host spleens was counted at different time intervals. The rate of isotope disappearance from the spleen was exponential to 0.5 per cent of the dose or less. In isologous recipients, the calculated half-life of labeled cells was 82 hours; in homologous recipients it was 40 hours; and in presensitized homologous hosts it was 18 hours. The technique is a highly reproducible, precise, and sensitive measure of transplantation immunity.
format Text
id pubmed-2137990
institution National Center for Biotechnology Information
language English
publishDate 1965
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21379902008-04-17 QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD Najarian, John S. Feldman, Joseph D. J Exp Med Article A technique for in vivo quantitation of transplantation immunity has been presented. Known numbers of H(3)-thymidine-labeled lymphoid cells were injected intravenously into isologous or homologous recipients. The total radioactivity in host spleens was counted at different time intervals. The rate of isotope disappearance from the spleen was exponential to 0.5 per cent of the dose or less. In isologous recipients, the calculated half-life of labeled cells was 82 hours; in homologous recipients it was 40 hours; and in presensitized homologous hosts it was 18 hours. The technique is a highly reproducible, precise, and sensitive measure of transplantation immunity. The Rockefeller University Press 1965-03-31 /pmc/articles/PMC2137990/ /pubmed/14276774 Text en Copyright © 1965 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Najarian, John S.
Feldman, Joseph D.
QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD
title QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD
title_full QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD
title_fullStr QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD
title_full_unstemmed QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD
title_short QUANTITATION OF TRANSPLANTATION IMMUNITY : I. METHOD
title_sort quantitation of transplantation immunity : i. method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137990/
https://www.ncbi.nlm.nih.gov/pubmed/14276774
work_keys_str_mv AT najarianjohns quantitationoftransplantationimmunityimethod
AT feldmanjosephd quantitationoftransplantationimmunityimethod

Ejemplares similares