THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION

A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit ant...

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Detalles Bibliográficos
Autores principales: Schmidt, Willard C., Moore, Dorothy J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138003/
https://www.ncbi.nlm.nih.gov/pubmed/14280441
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author Schmidt, Willard C.
Moore, Dorothy J.
author_facet Schmidt, Willard C.
Moore, Dorothy J.
author_sort Schmidt, Willard C.
collection PubMed
description A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented.
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spelling pubmed-21380032008-04-17 THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION Schmidt, Willard C. Moore, Dorothy J. J Exp Med Article A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented. The Rockefeller University Press 1965-05-01 /pmc/articles/PMC2138003/ /pubmed/14280441 Text en Copyright © 1965 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Schmidt, Willard C.
Moore, Dorothy J.
THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION
title THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION
title_full THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION
title_fullStr THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION
title_full_unstemmed THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION
title_short THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION
title_sort determination of antibody to group a streptococcal polysaccharide in human sera by hemagglutination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138003/
https://www.ncbi.nlm.nih.gov/pubmed/14280441
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