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ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT

At least 3 complement factors were found necessary for the conversion of the thermolabile intermediate complex EAC'1a,4,2a to a thermostable state. One of these factors is the earlier described β(1C)-globulin. The second, a heretofore unrecorded serum protein, β(1F)-globulin. The third factor h...

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Autores principales: Nilsson, Ulf R., Müller-Eberhard, Hans J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138056/
https://www.ncbi.nlm.nih.gov/pubmed/14316946
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author Nilsson, Ulf R.
Müller-Eberhard, Hans J.
author_facet Nilsson, Ulf R.
Müller-Eberhard, Hans J.
author_sort Nilsson, Ulf R.
collection PubMed
description At least 3 complement factors were found necessary for the conversion of the thermolabile intermediate complex EAC'1a,4,2a to a thermostable state. One of these factors is the earlier described β(1C)-globulin. The second, a heretofore unrecorded serum protein, β(1F)-globulin. The third factor has not yet been defined as a discrete serum protein entity. Kinetic experiments indicated that β(1C) reacted prior to β(1F), which in turn seemed to precede the third factor in the reaction sequence. Therefore, the 3 components were tentatively designated the third (C'3), the fifth (C'5), and the sixth (C'6) components of complement, respectively. A procedure was developed allowing the isolation of highly purified β(1C)-(C'3) and β(1F)-globulin (C'5) and of partially purified C'6. With respect to its function in immune hemolysis, β(1F)-globulin or C'5 was found to be closely dependent on the simultaneous presence of C'6. The hypothesis that C'5 and C'6 form a functional unit was supported by the finding that both components interact with each other in solution resulting in the formation of a complex. A similar complex was also found in fresh human serum.
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spelling pubmed-21380562008-04-17 ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT Nilsson, Ulf R. Müller-Eberhard, Hans J. J Exp Med Article At least 3 complement factors were found necessary for the conversion of the thermolabile intermediate complex EAC'1a,4,2a to a thermostable state. One of these factors is the earlier described β(1C)-globulin. The second, a heretofore unrecorded serum protein, β(1F)-globulin. The third factor has not yet been defined as a discrete serum protein entity. Kinetic experiments indicated that β(1C) reacted prior to β(1F), which in turn seemed to precede the third factor in the reaction sequence. Therefore, the 3 components were tentatively designated the third (C'3), the fifth (C'5), and the sixth (C'6) components of complement, respectively. A procedure was developed allowing the isolation of highly purified β(1C)-(C'3) and β(1F)-globulin (C'5) and of partially purified C'6. With respect to its function in immune hemolysis, β(1F)-globulin or C'5 was found to be closely dependent on the simultaneous presence of C'6. The hypothesis that C'5 and C'6 form a functional unit was supported by the finding that both components interact with each other in solution resulting in the formation of a complex. A similar complex was also found in fresh human serum. The Rockefeller University Press 1965-08-01 /pmc/articles/PMC2138056/ /pubmed/14316946 Text en Copyright © 1965 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Nilsson, Ulf R.
Müller-Eberhard, Hans J.
ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
title ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
title_full ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
title_fullStr ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
title_full_unstemmed ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
title_short ISOLATION OF β(1F)-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT
title_sort isolation of β(1f)-globulin from human serum and its characterization as the fifth component of complement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138056/
https://www.ncbi.nlm.nih.gov/pubmed/14316946
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