THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES

Rabbit γM- and γG-anti-S. typhimurium antibodies were isolated by combined immune specific and physical methods and some of their properties in immunological systems were measured. γM-Antibody was detectable at lower concentrations and revealed a higher specific activity than the γG-globulins. Indir...

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Detalles Bibliográficos
Autores principales: Robbins, John B., Kenny, Kathryn, Suter, Emanuel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138065/
https://www.ncbi.nlm.nih.gov/pubmed/14316950
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author Robbins, John B.
Kenny, Kathryn
Suter, Emanuel
author_facet Robbins, John B.
Kenny, Kathryn
Suter, Emanuel
author_sort Robbins, John B.
collection PubMed
description Rabbit γM- and γG-anti-S. typhimurium antibodies were isolated by combined immune specific and physical methods and some of their properties in immunological systems were measured. γM-Antibody was detectable at lower concentrations and revealed a higher specific activity than the γG-globulins. Indirect studies indicated that the γG-globulin was the more "avid" immunoglobulin. Treatment of the γM-globulin with the reducing agent 2-ME decreased but did not destroy the immune activity of the subunits. The results confirm the necessity of analysis of purified immunoglobulin antibodies to evaluate the significance of their biological properties secondary to their interaction with antigens.
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spelling pubmed-21380652008-04-17 THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES Robbins, John B. Kenny, Kathryn Suter, Emanuel J Exp Med Article Rabbit γM- and γG-anti-S. typhimurium antibodies were isolated by combined immune specific and physical methods and some of their properties in immunological systems were measured. γM-Antibody was detectable at lower concentrations and revealed a higher specific activity than the γG-globulins. Indirect studies indicated that the γG-globulin was the more "avid" immunoglobulin. Treatment of the γM-globulin with the reducing agent 2-ME decreased but did not destroy the immune activity of the subunits. The results confirm the necessity of analysis of purified immunoglobulin antibodies to evaluate the significance of their biological properties secondary to their interaction with antigens. The Rockefeller University Press 1965-08-01 /pmc/articles/PMC2138065/ /pubmed/14316950 Text en Copyright © 1965 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Robbins, John B.
Kenny, Kathryn
Suter, Emanuel
THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES
title THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES
title_full THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES
title_fullStr THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES
title_full_unstemmed THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES
title_short THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT γM- AND γG-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES
title_sort isolation and biological activities of rabbit γm- and γg-anti-salmonella typhimurium antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138065/
https://www.ncbi.nlm.nih.gov/pubmed/14316950
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