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GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE
Immunization of CBA and C57 mice with a branched, multichain synthetic polypeptide, poly (tyr,glu)-poly DL-ala--poly lys, ((T,G)-A--L), in Freund's complete adjuvant results in a tenfold or more difference in the antigen-binding capacity of sera from the two strains, although they respond equal...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1965
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138070/ https://www.ncbi.nlm.nih.gov/pubmed/5839284 |
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author | McDevitt, Hugh O. Sela, Michael |
author_facet | McDevitt, Hugh O. Sela, Michael |
author_sort | McDevitt, Hugh O. |
collection | PubMed |
description | Immunization of CBA and C57 mice with a branched, multichain synthetic polypeptide, poly (tyr,glu)-poly DL-ala--poly lys, ((T,G)-A--L), in Freund's complete adjuvant results in a tenfold or more difference in the antigen-binding capacity of sera from the two strains, although they respond equally to bovine serum albumin. Immunization of CBA x C57 F(1), F(1) x CBA, and F(1) x C57 mice reveals definite genetic control of the response to (T,G)-A--L, which appears to be due to a single major genetic factor, with perhaps one or more modifying factors. Immunization of CBA and C57 mice with (H,G)-A--L, a synthetic polypeptide in which histidine replaces tyrosine, gives the opposite result, CBA's respond and C57's do not. From this, it appears that the genetic control of the response to (T,G)-A--L is specific for the antigenic determinant. The implications of these results are discussed. |
format | Text |
id | pubmed-2138070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1965 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21380702008-04-17 GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE McDevitt, Hugh O. Sela, Michael J Exp Med Article Immunization of CBA and C57 mice with a branched, multichain synthetic polypeptide, poly (tyr,glu)-poly DL-ala--poly lys, ((T,G)-A--L), in Freund's complete adjuvant results in a tenfold or more difference in the antigen-binding capacity of sera from the two strains, although they respond equally to bovine serum albumin. Immunization of CBA x C57 F(1), F(1) x CBA, and F(1) x C57 mice reveals definite genetic control of the response to (T,G)-A--L, which appears to be due to a single major genetic factor, with perhaps one or more modifying factors. Immunization of CBA and C57 mice with (H,G)-A--L, a synthetic polypeptide in which histidine replaces tyrosine, gives the opposite result, CBA's respond and C57's do not. From this, it appears that the genetic control of the response to (T,G)-A--L is specific for the antigenic determinant. The implications of these results are discussed. The Rockefeller University Press 1965-09-01 /pmc/articles/PMC2138070/ /pubmed/5839284 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article McDevitt, Hugh O. Sela, Michael GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE |
title | GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE |
title_full | GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE |
title_fullStr | GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE |
title_full_unstemmed | GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE |
title_short | GENETIC CONTROL OF THE ANTIBODY RESPONSE : I. DEMONSTRATION OF DETERMINANT-SPECIFIC DIFFERENCES IN RESPONSE TO SYNTHETIC POLYPEPTIDE ANTIGENS IN TWO STRAINS OF INBRED MICE |
title_sort | genetic control of the antibody response : i. demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138070/ https://www.ncbi.nlm.nih.gov/pubmed/5839284 |
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