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FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE

Reticuloendothelial clearance of carbon particles is inhibited by injection of heat-aggregated albumin. The possibility that this inhibition might involve a serum component has been explored. Evidence that such a serum factor does participate in carbon clearance inhibition by heat-aggregated albumin...

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Detalles Bibliográficos
Autores principales: Normann, Sigurd J., Benditt, Earl P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138090/
https://www.ncbi.nlm.nih.gov/pubmed/5849236
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author Normann, Sigurd J.
Benditt, Earl P.
author_facet Normann, Sigurd J.
Benditt, Earl P.
author_sort Normann, Sigurd J.
collection PubMed
description Reticuloendothelial clearance of carbon particles is inhibited by injection of heat-aggregated albumin. The possibility that this inhibition might involve a serum component has been explored. Evidence that such a serum factor does participate in carbon clearance inhibition by heat-aggregated albumin derives from the fact that prior treatment of the carbon with homologous serum eliminates the blockade; and also, if serum previously absorbed with carbon is mixed with the carbon particles no restoration of "blockade" by albumin occurs. A method for extracting the component from serum using barium sulfate adsorption is described which allows concentration of the factor and sensitive assay for its presence. It is concluded that while large or multiple carbon injections may bring about a saturation of the phagocytic cell, this saturation masks an underlying factor which protects against acute inhibition of carbon phagocytosis by denatured albumin when subsaturating carbon doses are used.
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spelling pubmed-21380902008-04-17 FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE Normann, Sigurd J. Benditt, Earl P. J Exp Med Article Reticuloendothelial clearance of carbon particles is inhibited by injection of heat-aggregated albumin. The possibility that this inhibition might involve a serum component has been explored. Evidence that such a serum factor does participate in carbon clearance inhibition by heat-aggregated albumin derives from the fact that prior treatment of the carbon with homologous serum eliminates the blockade; and also, if serum previously absorbed with carbon is mixed with the carbon particles no restoration of "blockade" by albumin occurs. A method for extracting the component from serum using barium sulfate adsorption is described which allows concentration of the factor and sensitive assay for its presence. It is concluded that while large or multiple carbon injections may bring about a saturation of the phagocytic cell, this saturation masks an underlying factor which protects against acute inhibition of carbon phagocytosis by denatured albumin when subsaturating carbon doses are used. The Rockefeller University Press 1965-10-01 /pmc/articles/PMC2138090/ /pubmed/5849236 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Normann, Sigurd J.
Benditt, Earl P.
FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE
title FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE
title_full FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE
title_fullStr FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE
title_full_unstemmed FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE
title_short FUNCTION OF THE RETICULOENDOTHELIAL SYSTEM : II. PARTICIPATION OF A SERUM FACTOR IN CARBON CLEARANCE
title_sort function of the reticuloendothelial system : ii. participation of a serum factor in carbon clearance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138090/
https://www.ncbi.nlm.nih.gov/pubmed/5849236
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