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STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN

Leukocytic pyrogen previously reported to contain an essential protein moiety, appears to be a lipid-protein complex having a molecular weight in the range of 10,000 to 20,000. Evidence that it contains essential lipid includes its inactivation by Cu(++), its lability in alkaline solutions (pH 8.5 a...

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Detalles Bibliográficos
Autores principales: Rafter, Gale W., Cheuk, S. Fai, Krause, Donald W., Wood, W. Barry
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138150/
https://www.ncbi.nlm.nih.gov/pubmed/5937057
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author Rafter, Gale W.
Cheuk, S. Fai
Krause, Donald W.
Wood, W. Barry
author_facet Rafter, Gale W.
Cheuk, S. Fai
Krause, Donald W.
Wood, W. Barry
author_sort Rafter, Gale W.
collection PubMed
description Leukocytic pyrogen previously reported to contain an essential protein moiety, appears to be a lipid-protein complex having a molecular weight in the range of 10,000 to 20,000. Evidence that it contains essential lipid includes its inactivation by Cu(++), its lability in alkaline solutions (pH 8.5 and above), and its loss of pyrogenicity when extracted with acid-isooctane. Its solubility in 66% methanol, and the enhancing action of ethanol in freeing it from sonicated cells, suggest the presence of exposed lipid groups at its surface. Once the complex is separated from other proteins, its biological activity is readily destroyed. Although the lipid component is presumed to contain unesterified fatty acid(s), its precise composition is unknown. The finding of lipid in the active complex is in keeping with the hypothesis that the pyrogen is derived from leukocytic membranes.
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spelling pubmed-21381502008-04-17 STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN Rafter, Gale W. Cheuk, S. Fai Krause, Donald W. Wood, W. Barry J Exp Med Article Leukocytic pyrogen previously reported to contain an essential protein moiety, appears to be a lipid-protein complex having a molecular weight in the range of 10,000 to 20,000. Evidence that it contains essential lipid includes its inactivation by Cu(++), its lability in alkaline solutions (pH 8.5 and above), and its loss of pyrogenicity when extracted with acid-isooctane. Its solubility in 66% methanol, and the enhancing action of ethanol in freeing it from sonicated cells, suggest the presence of exposed lipid groups at its surface. Once the complex is separated from other proteins, its biological activity is readily destroyed. Although the lipid component is presumed to contain unesterified fatty acid(s), its precise composition is unknown. The finding of lipid in the active complex is in keeping with the hypothesis that the pyrogen is derived from leukocytic membranes. The Rockefeller University Press 1966-02-28 /pmc/articles/PMC2138150/ /pubmed/5937057 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rafter, Gale W.
Cheuk, S. Fai
Krause, Donald W.
Wood, W. Barry
STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN
title STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN
title_full STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN
title_fullStr STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN
title_full_unstemmed STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN
title_short STUDIES ON THE PATHOGENESIS OF FEVER : XIV. FURTHER OBSERVATIONS ON THE CHEMISTRY OF LEUKOCYTIC PYROGEN
title_sort studies on the pathogenesis of fever : xiv. further observations on the chemistry of leukocytic pyrogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138150/
https://www.ncbi.nlm.nih.gov/pubmed/5937057
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