CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM

Rabbit antibody capable of neutralizing the pressor activity of the enzyme renin, derived from hog kidneys, was characterized with respect to type and capacity to react serologically. Three antirenin antibodies were detected, both a precipitating and nonprecipitating, neutralizing antibody as well a...

Descripción completa

Detalles Bibliográficos
Autores principales: Warren, Bert, Johnson, Arthur G., Hoobler, S. W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138182/
https://www.ncbi.nlm.nih.gov/pubmed/5941784
_version_ 1782143503573188608
author Warren, Bert
Johnson, Arthur G.
Hoobler, S. W.
author_facet Warren, Bert
Johnson, Arthur G.
Hoobler, S. W.
author_sort Warren, Bert
collection PubMed
description Rabbit antibody capable of neutralizing the pressor activity of the enzyme renin, derived from hog kidneys, was characterized with respect to type and capacity to react serologically. Three antirenin antibodies were detected, both a precipitating and nonprecipitating, neutralizing antibody as well as a neutralizing antibody capable of being absorbed by inactivated renin. The precipitating, neutralizing antibody was purified and used successfully in an assay for hog renin by means of a hemagglutination inhibition test. By immunofluorescent methods utilizing the purified antiserum, renin was found to be localized in the macula densa of the distal convoluted tubules and the juxtaglomerular cells of the afferent arteriole in both hog and dog kidneys.
format Text
id pubmed-2138182
institution National Center for Biotechnology Information
language English
publishDate 1966
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21381822008-04-17 CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM Warren, Bert Johnson, Arthur G. Hoobler, S. W. J Exp Med Article Rabbit antibody capable of neutralizing the pressor activity of the enzyme renin, derived from hog kidneys, was characterized with respect to type and capacity to react serologically. Three antirenin antibodies were detected, both a precipitating and nonprecipitating, neutralizing antibody as well as a neutralizing antibody capable of being absorbed by inactivated renin. The precipitating, neutralizing antibody was purified and used successfully in an assay for hog renin by means of a hemagglutination inhibition test. By immunofluorescent methods utilizing the purified antiserum, renin was found to be localized in the macula densa of the distal convoluted tubules and the juxtaglomerular cells of the afferent arteriole in both hog and dog kidneys. The Rockefeller University Press 1966-06-01 /pmc/articles/PMC2138182/ /pubmed/5941784 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Warren, Bert
Johnson, Arthur G.
Hoobler, S. W.
CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM
title CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM
title_full CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM
title_fullStr CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM
title_full_unstemmed CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM
title_short CHARACTERIZATION OF THE RENIN-ANTIRENIN SYSTEM
title_sort characterization of the renin-antirenin system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138182/
https://www.ncbi.nlm.nih.gov/pubmed/5941784
work_keys_str_mv AT warrenbert characterizationofthereninantireninsystem
AT johnsonarthurg characterizationofthereninantireninsystem
AT hooblersw characterizationofthereninantireninsystem

Ejemplares similares