STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN

A study of the kinetics of RNA and DNA synthesis in PWM-stimulated lymphocytes revealed that RNA synthesis preceded the onset of DNA synthesis by approximately 24 hr and that DNA synthesis and transformation was maximal between 66 to 78 hr. Histochemical and radioautographic studies on PWM stimulate...

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Detalles Bibliográficos
Autores principales: Chessin, Lawrence N., Börjeson, Jan, Welsh, Patricia D., Douglas, Steven D., Cooper, Herbert L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138260/
https://www.ncbi.nlm.nih.gov/pubmed/5926301
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author Chessin, Lawrence N.
Börjeson, Jan
Welsh, Patricia D.
Douglas, Steven D.
Cooper, Herbert L.
author_facet Chessin, Lawrence N.
Börjeson, Jan
Welsh, Patricia D.
Douglas, Steven D.
Cooper, Herbert L.
author_sort Chessin, Lawrence N.
collection PubMed
description A study of the kinetics of RNA and DNA synthesis in PWM-stimulated lymphocytes revealed that RNA synthesis preceded the onset of DNA synthesis by approximately 24 hr and that DNA synthesis and transformation was maximal between 66 to 78 hr. Histochemical and radioautographic studies on PWM stimulated cultures indicated that at 72 hr 50 to 60% of the cell population had been transformed by PWM, and that a distinct cell type bearing cytologic resemblance to the early plasma cell had emerged. The RNA sedimentation profile for newly synthesized RNA in PWM-stimulated cells showed that a large peak of 45 to 50 S material was formed after 24 and 40 hr. PWM thus produces a distinctive transformation of human peripheral blood lymphocytes.
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spelling pubmed-21382602008-04-17 STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN Chessin, Lawrence N. Börjeson, Jan Welsh, Patricia D. Douglas, Steven D. Cooper, Herbert L. J Exp Med Article A study of the kinetics of RNA and DNA synthesis in PWM-stimulated lymphocytes revealed that RNA synthesis preceded the onset of DNA synthesis by approximately 24 hr and that DNA synthesis and transformation was maximal between 66 to 78 hr. Histochemical and radioautographic studies on PWM stimulated cultures indicated that at 72 hr 50 to 60% of the cell population had been transformed by PWM, and that a distinct cell type bearing cytologic resemblance to the early plasma cell had emerged. The RNA sedimentation profile for newly synthesized RNA in PWM-stimulated cells showed that a large peak of 45 to 50 S material was formed after 24 and 40 hr. PWM thus produces a distinctive transformation of human peripheral blood lymphocytes. The Rockefeller University Press 1966-10-31 /pmc/articles/PMC2138260/ /pubmed/5926301 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Chessin, Lawrence N.
Börjeson, Jan
Welsh, Patricia D.
Douglas, Steven D.
Cooper, Herbert L.
STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN
title STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN
title_full STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN
title_fullStr STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN
title_full_unstemmed STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN
title_short STUDIES ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO : II. MORPHOLOGICAL AND BIOCHEMICAL STUDIES ON THE TRANSFORMATION OF LYMPHOCYTES BY POKEWEED MITOGEN
title_sort studies on human peripheral blood lymphocytes in vitro : ii. morphological and biochemical studies on the transformation of lymphocytes by pokeweed mitogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138260/
https://www.ncbi.nlm.nih.gov/pubmed/5926301
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