MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT

The mast cell-rupturing component present in the lysosomes of rabbit exudate PMN neutrophil leukocytes has been identified and some of its physical and chemical properties have been described. The active agent is a low molecular weight (1200 to 2400) polypeptide containing a relatively large proport...

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Detalles Bibliográficos
Autores principales: Seegers, Winnifred, Janoff, Aaron
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138271/
https://www.ncbi.nlm.nih.gov/pubmed/5926298
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author Seegers, Winnifred
Janoff, Aaron
author_facet Seegers, Winnifred
Janoff, Aaron
author_sort Seegers, Winnifred
collection PubMed
description The mast cell-rupturing component present in the lysosomes of rabbit exudate PMN neutrophil leukocytes has been identified and some of its physical and chemical properties have been described. The active agent is a low molecular weight (1200 to 2400) polypeptide containing a relatively large proportion of the basic amino acid, arginine. It is thermostable and dialyzable, and does not cause contraction of the isolated guinea pig ileum. The mast cell-rupturing activity of the agent is destroyed by trypsin. A second permeability factor with a larger molecular weight is present in crude extracts of PMN granules. Although this substance does not lyse mast cells, it is capable of evoking delayed permeability responses in rabbit skin.
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spelling pubmed-21382712008-04-17 MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT Seegers, Winnifred Janoff, Aaron J Exp Med Article The mast cell-rupturing component present in the lysosomes of rabbit exudate PMN neutrophil leukocytes has been identified and some of its physical and chemical properties have been described. The active agent is a low molecular weight (1200 to 2400) polypeptide containing a relatively large proportion of the basic amino acid, arginine. It is thermostable and dialyzable, and does not cause contraction of the isolated guinea pig ileum. The mast cell-rupturing activity of the agent is destroyed by trypsin. A second permeability factor with a larger molecular weight is present in crude extracts of PMN granules. Although this substance does not lyse mast cells, it is capable of evoking delayed permeability responses in rabbit skin. The Rockefeller University Press 1966-10-31 /pmc/articles/PMC2138271/ /pubmed/5926298 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Seegers, Winnifred
Janoff, Aaron
MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT
title MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT
title_full MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT
title_fullStr MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT
title_full_unstemmed MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT
title_short MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : VI. PARTIAL PURIFICATION AND CHARACTERIZATION OF A MAST CELL-RUPTURING COMPONENT
title_sort mediators of inflammation in leukocyte lysosomes : vi. partial purification and characterization of a mast cell-rupturing component
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138271/
https://www.ncbi.nlm.nih.gov/pubmed/5926298
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