PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO

A method for the primary stimulation and measurement of antibody production to sheep red blood cells in vitro has been described. In this system when mouse spleen cells are incubated with sheep erythrocytes large complement-dependent plaques are formed. The number and size of plaques increases from...

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Detalles Bibliográficos
Autores principales: Robinson, W. A., Marbrook, J., Diener, E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138321/
https://www.ncbi.nlm.nih.gov/pubmed/4165744
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author Robinson, W. A.
Marbrook, J.
Diener, E.
author_facet Robinson, W. A.
Marbrook, J.
Diener, E.
author_sort Robinson, W. A.
collection PubMed
description A method for the primary stimulation and measurement of antibody production to sheep red blood cells in vitro has been described. In this system when mouse spleen cells are incubated with sheep erythrocytes large complement-dependent plaques are formed. The number and size of plaques increases from day 1 to day 3 of incubation with an average of 4.4 plaque areas per 1 x 10(6) cells plated at day 3. There is a linear relationship between the number of spleen cells plated and the number of plaques formed. Plaque formation is inhibited by colchicine, actinomycin D, and rabbit anti-mouse globulin. This system offers a possible means for the direct in vitro measurement of the number of cells in a population susceptible to antigenic stimulation by sheep erythrocytes.
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spelling pubmed-21383212008-04-17 PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO Robinson, W. A. Marbrook, J. Diener, E. J Exp Med Article A method for the primary stimulation and measurement of antibody production to sheep red blood cells in vitro has been described. In this system when mouse spleen cells are incubated with sheep erythrocytes large complement-dependent plaques are formed. The number and size of plaques increases from day 1 to day 3 of incubation with an average of 4.4 plaque areas per 1 x 10(6) cells plated at day 3. There is a linear relationship between the number of spleen cells plated and the number of plaques formed. Plaque formation is inhibited by colchicine, actinomycin D, and rabbit anti-mouse globulin. This system offers a possible means for the direct in vitro measurement of the number of cells in a population susceptible to antigenic stimulation by sheep erythrocytes. The Rockefeller University Press 1967-08-01 /pmc/articles/PMC2138321/ /pubmed/4165744 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Robinson, W. A.
Marbrook, J.
Diener, E.
PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO
title PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO
title_full PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO
title_fullStr PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO
title_full_unstemmed PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO
title_short PRIMARY STIMULATION AND MEASUREMENT OF ANTIBODY PRODUCTION TO SHEEP RED BLOOD CELLS IN VITRO
title_sort primary stimulation and measurement of antibody production to sheep red blood cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138321/
https://www.ncbi.nlm.nih.gov/pubmed/4165744
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AT marbrookj primarystimulationandmeasurementofantibodyproductiontosheepredbloodcellsinvitro
AT dienere primarystimulationandmeasurementofantibodyproductiontosheepredbloodcellsinvitro

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