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STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D

Preparations of streptococcal DNAse D with high specific activity and free of other streptococcal nucleases have been obtained by zone electrophoresis and column chromatography. Antisera prepared by injecting rabbits with such preparations specifically neutralize the activity of this enzyme. As with...

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Detalles Bibliográficos
Autores principales: Wannamaker, Lewis W., Hayes, Barbara, Yasmineh, Walid
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138379/
https://www.ncbi.nlm.nih.gov/pubmed/4962268
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author Wannamaker, Lewis W.
Hayes, Barbara
Yasmineh, Walid
author_facet Wannamaker, Lewis W.
Hayes, Barbara
Yasmineh, Walid
author_sort Wannamaker, Lewis W.
collection PubMed
description Preparations of streptococcal DNAse D with high specific activity and free of other streptococcal nucleases have been obtained by zone electrophoresis and column chromatography. Antisera prepared by injecting rabbits with such preparations specifically neutralize the activity of this enzyme. As with DNAse B, preparations of DNAse D regularly exhibit ribonuclease activity. For both B and D enzymes, the order of substrate preference is thymus DNA, yeast RNA, bacterial RNA; but the specific activity of the D enzyme is higher than that of the B enzyme with respect to thymus DNA and lower with respect to bacterial RNA. Both the deoxyribonuclease and the ribonuclease activities exhibited by preparations of both enzymes are inhibited by bacterial RNA, but approximately 100-fold greater concentrations of bacterial RNA are required to achieve inhibition of the deoxyribonuclease activity of the D enzyme equivalent to the inhibition of the B enzyme. The deoxyribonuclease activity of the D enzyme is also inhibited by yeast RNA, but even larger amounts are required. These observations indicate that the D enzyme is immunologically distinct from the other streptococcal nucleases and that it differs quantitatively from the B enzyme with respect to relative specific activities on different substrates and behavior in the presence of the bacterial ribonucleic acid inhibitor.
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spelling pubmed-21383792008-04-17 STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D Wannamaker, Lewis W. Hayes, Barbara Yasmineh, Walid J Exp Med Article Preparations of streptococcal DNAse D with high specific activity and free of other streptococcal nucleases have been obtained by zone electrophoresis and column chromatography. Antisera prepared by injecting rabbits with such preparations specifically neutralize the activity of this enzyme. As with DNAse B, preparations of DNAse D regularly exhibit ribonuclease activity. For both B and D enzymes, the order of substrate preference is thymus DNA, yeast RNA, bacterial RNA; but the specific activity of the D enzyme is higher than that of the B enzyme with respect to thymus DNA and lower with respect to bacterial RNA. Both the deoxyribonuclease and the ribonuclease activities exhibited by preparations of both enzymes are inhibited by bacterial RNA, but approximately 100-fold greater concentrations of bacterial RNA are required to achieve inhibition of the deoxyribonuclease activity of the D enzyme equivalent to the inhibition of the B enzyme. The deoxyribonuclease activity of the D enzyme is also inhibited by yeast RNA, but even larger amounts are required. These observations indicate that the D enzyme is immunologically distinct from the other streptococcal nucleases and that it differs quantitatively from the B enzyme with respect to relative specific activities on different substrates and behavior in the presence of the bacterial ribonucleic acid inhibitor. The Rockefeller University Press 1967-09-01 /pmc/articles/PMC2138379/ /pubmed/4962268 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wannamaker, Lewis W.
Hayes, Barbara
Yasmineh, Walid
STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D
title STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D
title_full STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D
title_fullStr STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D
title_full_unstemmed STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D
title_short STREPTOCOCCAL NUCLEASES : II. CHARACTERIZATION OF DNASE D
title_sort streptococcal nucleases : ii. characterization of dnase d
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138379/
https://www.ncbi.nlm.nih.gov/pubmed/4962268
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