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AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37°C, and injection into rabbits ren...
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1967
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138405/ https://www.ncbi.nlm.nih.gov/pubmed/4863132 |
| Sumario: | A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37°C, and injection into rabbits rendered refractory to endotoxin. The validity of the assay was confirmed by formal statistical methods. A simplified assay method is also described which lacks full statistical validity, but is more suitable for routine use. |
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