AN ASSAY METHOD FOR LEUKOCYTE PYROGEN

A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37°C, and injection into rabbits ren...

Descripción completa

Detalles Bibliográficos
Autor principal: Murphy, P. A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138405/
https://www.ncbi.nlm.nih.gov/pubmed/4863132
_version_ 1782143555664347136
author Murphy, P. A.
author_facet Murphy, P. A.
author_sort Murphy, P. A.
collection PubMed
description A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37°C, and injection into rabbits rendered refractory to endotoxin. The validity of the assay was confirmed by formal statistical methods. A simplified assay method is also described which lacks full statistical validity, but is more suitable for routine use.
format Text
id pubmed-2138405
institution National Center for Biotechnology Information
language English
publishDate 1967
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21384052008-04-17 AN ASSAY METHOD FOR LEUKOCYTE PYROGEN Murphy, P. A. J Exp Med Article A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37°C, and injection into rabbits rendered refractory to endotoxin. The validity of the assay was confirmed by formal statistical methods. A simplified assay method is also described which lacks full statistical validity, but is more suitable for routine use. The Rockefeller University Press 1967-10-31 /pmc/articles/PMC2138405/ /pubmed/4863132 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Murphy, P. A.
AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
title AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
title_full AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
title_fullStr AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
title_full_unstemmed AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
title_short AN ASSAY METHOD FOR LEUKOCYTE PYROGEN
title_sort assay method for leukocyte pyrogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138405/
https://www.ncbi.nlm.nih.gov/pubmed/4863132
work_keys_str_mv AT murphypa anassaymethodforleukocytepyrogen
AT murphypa assaymethodforleukocytepyrogen

Ejemplares similares