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CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE

Articular cartilage fragments were added to monolayer cultures of synovial membrane cells. After 3 wk of incubation, the cartilage fragments were examined histologically for metachromasia and basophilia, and for fluorescent staining using a rabbit antiserum to cartilage protein-polysaccharide. Carti...

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Detalles Bibliográficos
Autores principales: Hamerman, David, Janis, Rosamond, Smith, Carol
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138422/
https://www.ncbi.nlm.nih.gov/pubmed/4168366
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author Hamerman, David
Janis, Rosamond
Smith, Carol
author_facet Hamerman, David
Janis, Rosamond
Smith, Carol
author_sort Hamerman, David
collection PubMed
description Articular cartilage fragments were added to monolayer cultures of synovial membrane cells. After 3 wk of incubation, the cartilage fragments were examined histologically for metachromasia and basophilia, and for fluorescent staining using a rabbit antiserum to cartilage protein-polysaccharide. Cartilage incubated with cells derived from rheumatoid synovial membranes showed striking loss of metachromasia and basophilia as well as diminished to absent fluorescent staining. Cartilage fragments incubated with cells from normal synovia, or with cells from the synovial membrane of a patient with Reiter's syndrome, did not show these changes and resembled control cartilage incubated in tissue culture medium alone. It appears, therefore, that rheumatoid synovial cells in tissue culture are able to deplete the matrix of articular cartilage.
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spelling pubmed-21384222008-04-17 CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE Hamerman, David Janis, Rosamond Smith, Carol J Exp Med Article Articular cartilage fragments were added to monolayer cultures of synovial membrane cells. After 3 wk of incubation, the cartilage fragments were examined histologically for metachromasia and basophilia, and for fluorescent staining using a rabbit antiserum to cartilage protein-polysaccharide. Cartilage incubated with cells derived from rheumatoid synovial membranes showed striking loss of metachromasia and basophilia as well as diminished to absent fluorescent staining. Cartilage fragments incubated with cells from normal synovia, or with cells from the synovial membrane of a patient with Reiter's syndrome, did not show these changes and resembled control cartilage incubated in tissue culture medium alone. It appears, therefore, that rheumatoid synovial cells in tissue culture are able to deplete the matrix of articular cartilage. The Rockefeller University Press 1967-11-30 /pmc/articles/PMC2138422/ /pubmed/4168366 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hamerman, David
Janis, Rosamond
Smith, Carol
CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
title CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
title_full CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
title_fullStr CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
title_full_unstemmed CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
title_short CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
title_sort cartilage matrix depletion by rheumatoid synovial cells in tissue culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138422/
https://www.ncbi.nlm.nih.gov/pubmed/4168366
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