STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN

Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical prop...

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Detalles Bibliográficos
Autores principales: Kozak, Marilyn Sue, Hahn, Helmut H., Lennarz, William J., Wood, W. Barry
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138451/
https://www.ncbi.nlm.nih.gov/pubmed/5635382
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author Kozak, Marilyn Sue
Hahn, Helmut H.
Lennarz, William J.
Wood, W. Barry
author_facet Kozak, Marilyn Sue
Hahn, Helmut H.
Lennarz, William J.
Wood, W. Barry
author_sort Kozak, Marilyn Sue
collection PubMed
description Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane.
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spelling pubmed-21384512008-04-17 STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN Kozak, Marilyn Sue Hahn, Helmut H. Lennarz, William J. Wood, W. Barry J Exp Med Article Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane. The Rockefeller University Press 1968-01-31 /pmc/articles/PMC2138451/ /pubmed/5635382 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kozak, Marilyn Sue
Hahn, Helmut H.
Lennarz, William J.
Wood, W. Barry
STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN
title STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN
title_full STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN
title_fullStr STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN
title_full_unstemmed STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN
title_short STUDIES ON THE PATHOGENESIS OF FEVER : XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN
title_sort studies on the pathogenesis of fever : xvi. purification and further chemical characterization of granulocytic pyrogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138451/
https://www.ncbi.nlm.nih.gov/pubmed/5635382
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