Cargando…

RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE

White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep...

Descripción completa

Detalles Bibliográficos
Autores principales: Wakefield, J. D., Thorbecke, G. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138516/
https://www.ncbi.nlm.nih.gov/pubmed/5662014
_version_ 1782143581515939840
author Wakefield, J. D.
Thorbecke, G. J.
author_facet Wakefield, J. D.
Thorbecke, G. J.
author_sort Wakefield, J. D.
collection PubMed
description White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing. White pulp was a much richer source of responsive cells than was whole spleen.
format Text
id pubmed-2138516
institution National Center for Biotechnology Information
language English
publishDate 1968
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21385162008-04-17 RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE Wakefield, J. D. Thorbecke, G. J. J Exp Med Article White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing. White pulp was a much richer source of responsive cells than was whole spleen. The Rockefeller University Press 1968-07-01 /pmc/articles/PMC2138516/ /pubmed/5662014 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wakefield, J. D.
Thorbecke, G. J.
RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
title RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
title_full RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
title_fullStr RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
title_full_unstemmed RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
title_short RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
title_sort relationship of germinal centers in lymphoid tissue to immunological memory : ii. the detection of primed cells and their proliferation upon cell transfer to lethally irradiated syngeneic mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138516/
https://www.ncbi.nlm.nih.gov/pubmed/5662014
work_keys_str_mv AT wakefieldjd relationshipofgerminalcentersinlymphoidtissuetoimmunologicalmemoryiithedetectionofprimedcellsandtheirproliferationuponcelltransfertolethallyirradiatedsyngeneicmice
AT thorbeckegj relationshipofgerminalcentersinlymphoidtissuetoimmunologicalmemoryiithedetectionofprimedcellsandtheirproliferationuponcelltransfertolethallyirradiatedsyngeneicmice