Cargando…
RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE
White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1968
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138516/ https://www.ncbi.nlm.nih.gov/pubmed/5662014 |
_version_ | 1782143581515939840 |
---|---|
author | Wakefield, J. D. Thorbecke, G. J. |
author_facet | Wakefield, J. D. Thorbecke, G. J. |
author_sort | Wakefield, J. D. |
collection | PubMed |
description | White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing. White pulp was a much richer source of responsive cells than was whole spleen. |
format | Text |
id | pubmed-2138516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1968 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21385162008-04-17 RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE Wakefield, J. D. Thorbecke, G. J. J Exp Med Article White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing. White pulp was a much richer source of responsive cells than was whole spleen. The Rockefeller University Press 1968-07-01 /pmc/articles/PMC2138516/ /pubmed/5662014 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Wakefield, J. D. Thorbecke, G. J. RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE |
title | RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE |
title_full | RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE |
title_fullStr | RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE |
title_full_unstemmed | RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE |
title_short | RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY : II. THE DETECTION OF PRIMED CELLS AND THEIR PROLIFERATION UPON CELL TRANSFER TO LETHALLY IRRADIATED SYNGENEIC MICE |
title_sort | relationship of germinal centers in lymphoid tissue to immunological memory : ii. the detection of primed cells and their proliferation upon cell transfer to lethally irradiated syngeneic mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138516/ https://www.ncbi.nlm.nih.gov/pubmed/5662014 |
work_keys_str_mv | AT wakefieldjd relationshipofgerminalcentersinlymphoidtissuetoimmunologicalmemoryiithedetectionofprimedcellsandtheirproliferationuponcelltransfertolethallyirradiatedsyngeneicmice AT thorbeckegj relationshipofgerminalcentersinlymphoidtissuetoimmunologicalmemoryiithedetectionofprimedcellsandtheirproliferationuponcelltransfertolethallyirradiatedsyngeneicmice |