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THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE

A method has been described for the purification and isolation of the second component of complement (C'2) from human serum. The protein is a β(1)-globulin with an approximate molecular weight of 117,000. Immunochemical analysis using a variety of specific antisera, including a monospecific ant...

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Detalles Bibliográficos
Autores principales: Polley, Margaret J., Müller-Eberhard, Hans J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138531/
https://www.ncbi.nlm.nih.gov/pubmed/5666963
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author Polley, Margaret J.
Müller-Eberhard, Hans J.
author_facet Polley, Margaret J.
Müller-Eberhard, Hans J.
author_sort Polley, Margaret J.
collection PubMed
description A method has been described for the purification and isolation of the second component of complement (C'2) from human serum. The protein is a β(1)-globulin with an approximate molecular weight of 117,000. Immunochemical analysis using a variety of specific antisera, including a monospecific antiserum to the isolated protein, indicate that the C'2 protein represents a heretofore unrecognized human serum constituent. Isolated C'2 contained 2 x 10(9) "effective molecules" per microgram and 1000 hemolytically active C'2 molecules were required to produce a single hemolytically effective C'2 site on erythrocytes undergoing immune cytolysis. C'1 esterase treatment of C'2 resulted in reduction of both its electrophoretic mobility and its molecular size, the latter observation indicating fragmentation of the molecule. Direct evidence was presented for the physical presence of C'2 as an integral part of the enzyme C'3 convertase.
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spelling pubmed-21385312008-04-17 THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE Polley, Margaret J. Müller-Eberhard, Hans J. J Exp Med Article A method has been described for the purification and isolation of the second component of complement (C'2) from human serum. The protein is a β(1)-globulin with an approximate molecular weight of 117,000. Immunochemical analysis using a variety of specific antisera, including a monospecific antiserum to the isolated protein, indicate that the C'2 protein represents a heretofore unrecognized human serum constituent. Isolated C'2 contained 2 x 10(9) "effective molecules" per microgram and 1000 hemolytically active C'2 molecules were required to produce a single hemolytically effective C'2 site on erythrocytes undergoing immune cytolysis. C'1 esterase treatment of C'2 resulted in reduction of both its electrophoretic mobility and its molecular size, the latter observation indicating fragmentation of the molecule. Direct evidence was presented for the physical presence of C'2 as an integral part of the enzyme C'3 convertase. The Rockefeller University Press 1968-09-01 /pmc/articles/PMC2138531/ /pubmed/5666963 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Polley, Margaret J.
Müller-Eberhard, Hans J.
THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE
title THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE
title_full THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE
title_fullStr THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE
title_full_unstemmed THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE
title_short THE SECOND COMPONENT OF HUMAN COMPLEMENT: ITS ISOLATION, FRAGMENTATION BY C'1 ESTERASE, AND INCORPORATION INTO C'3 CONVERTASE
title_sort second component of human complement: its isolation, fragmentation by c'1 esterase, and incorporation into c'3 convertase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138531/
https://www.ncbi.nlm.nih.gov/pubmed/5666963
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