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SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO

Isolated segments of human colon and to a lesser extent ileum were capable of synthesizing hemolytically active C'1. This conclusion was based on the following evidence: After elimination of C'1 from tissue with EDTA, we found that segments of the intestinal tract in short-term organ cultu...

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Detalles Bibliográficos
Autores principales: Colten, Harvey R., Gordon, James M., Borsos, Tibor, Rapp, Herbert J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138552/
https://www.ncbi.nlm.nih.gov/pubmed/5675435
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author Colten, Harvey R.
Gordon, James M.
Borsos, Tibor
Rapp, Herbert J.
author_facet Colten, Harvey R.
Gordon, James M.
Borsos, Tibor
Rapp, Herbert J.
author_sort Colten, Harvey R.
collection PubMed
description Isolated segments of human colon and to a lesser extent ileum were capable of synthesizing hemolytically active C'1. This conclusion was based on the following evidence: After elimination of C'1 from tissue with EDTA, we found that segments of the intestinal tract in short-term organ culture showed a 50–1000-fold increase in C'1 activity. The rate of production of C'1 in human intestine was highly temperature dependent; C'1 production was reversibly inhibited by puromycin and actinomycin D. Furthermore, (14)C-labeled amino acids were incorporated into molecules which behaved like C'1. No significant C'1(hu) synthesis was observed in isolated segments of jejunum, stomach, liver, kidney, lung, spleen, lymph node, and thymus.
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spelling pubmed-21385522008-04-17 SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO Colten, Harvey R. Gordon, James M. Borsos, Tibor Rapp, Herbert J. J Exp Med Article Isolated segments of human colon and to a lesser extent ileum were capable of synthesizing hemolytically active C'1. This conclusion was based on the following evidence: After elimination of C'1 from tissue with EDTA, we found that segments of the intestinal tract in short-term organ culture showed a 50–1000-fold increase in C'1 activity. The rate of production of C'1 in human intestine was highly temperature dependent; C'1 production was reversibly inhibited by puromycin and actinomycin D. Furthermore, (14)C-labeled amino acids were incorporated into molecules which behaved like C'1. No significant C'1(hu) synthesis was observed in isolated segments of jejunum, stomach, liver, kidney, lung, spleen, lymph node, and thymus. The Rockefeller University Press 1968-10-01 /pmc/articles/PMC2138552/ /pubmed/5675435 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Colten, Harvey R.
Gordon, James M.
Borsos, Tibor
Rapp, Herbert J.
SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO
title SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO
title_full SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO
title_fullStr SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO
title_full_unstemmed SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO
title_short SYNTHESIS OF THE FIRST COMPONENT OF HUMAN COMPLEMENT IN VITRO
title_sort synthesis of the first component of human complement in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138552/
https://www.ncbi.nlm.nih.gov/pubmed/5675435
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