Cargando…

MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES

The present study demonstrates that a granule fraction derived from human polymorphonuclear leukocytes possesses elastinolytic activity, and that the latter can be separated from the collagenase present in these cells. Properties of the human leukocyte elastase differ sufficiently from those of panc...

Descripción completa

Detalles Bibliográficos
Autores principales: Janoff, A., Scherer, J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138566/
https://www.ncbi.nlm.nih.gov/pubmed/5303065
_version_ 1782143593231679488
author Janoff, A.
Scherer, J.
author_facet Janoff, A.
Scherer, J.
author_sort Janoff, A.
collection PubMed
description The present study demonstrates that a granule fraction derived from human polymorphonuclear leukocytes possesses elastinolytic activity, and that the latter can be separated from the collagenase present in these cells. Properties of the human leukocyte elastase differ sufficiently from those of pancreatic elastases of different species as to suggest that the former enzyme is a distinct and separate entity. Thus, soybean trypsin inhibitor and salivary kallikrein inhibitor (Trasylol) fail to inhibit elastolysis by the pancreatic enzyme, but do inhibit the leukocyte elastinolytic agent. Elastolysis by human leukocyte granule extract does not show significant salt inhibition, whereas that catalyzed by pancreatic elastase is markedly reduced when ionic strength is increased to physiological levels. The leukocyte granule extract is at least 10 times more resistant to serum elastase inhibitor than is the purified pancreatic enzyme. Both enzymes show optimal elastolysis above pH 8.5, but the leukocyte factor still retains 50% of its maximal elastolytic activity at pH 6–7; whereas the activity of the pancreatic enzyme falls to 10% or less of its maximal value under the same conditions. The foregoing characteristics of the human leukocyte elastase suggest that it, rather than pancreatic (serum) elastase, may mediate pathological elastolysis during acute arteritis in man. In keeping with this suggestion, the present experiments also show that elastica staining of human arterial vessels is reduced by incubation of tissues with human leukocyte granule extracts in vitro.
format Text
id pubmed-2138566
institution National Center for Biotechnology Information
language English
publishDate 1968
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21385662008-04-17 MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES Janoff, A. Scherer, J. J Exp Med Article The present study demonstrates that a granule fraction derived from human polymorphonuclear leukocytes possesses elastinolytic activity, and that the latter can be separated from the collagenase present in these cells. Properties of the human leukocyte elastase differ sufficiently from those of pancreatic elastases of different species as to suggest that the former enzyme is a distinct and separate entity. Thus, soybean trypsin inhibitor and salivary kallikrein inhibitor (Trasylol) fail to inhibit elastolysis by the pancreatic enzyme, but do inhibit the leukocyte elastinolytic agent. Elastolysis by human leukocyte granule extract does not show significant salt inhibition, whereas that catalyzed by pancreatic elastase is markedly reduced when ionic strength is increased to physiological levels. The leukocyte granule extract is at least 10 times more resistant to serum elastase inhibitor than is the purified pancreatic enzyme. Both enzymes show optimal elastolysis above pH 8.5, but the leukocyte factor still retains 50% of its maximal elastolytic activity at pH 6–7; whereas the activity of the pancreatic enzyme falls to 10% or less of its maximal value under the same conditions. The foregoing characteristics of the human leukocyte elastase suggest that it, rather than pancreatic (serum) elastase, may mediate pathological elastolysis during acute arteritis in man. In keeping with this suggestion, the present experiments also show that elastica staining of human arterial vessels is reduced by incubation of tissues with human leukocyte granule extracts in vitro. The Rockefeller University Press 1968-10-31 /pmc/articles/PMC2138566/ /pubmed/5303065 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Janoff, A.
Scherer, J.
MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES
title MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES
title_full MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES
title_fullStr MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES
title_full_unstemmed MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES
title_short MEDIATORS OF INFLAMMATION IN LEUKOCYTE LYSOSOMES : IX. ELASTINOLYTIC ACTIVITY IN GRANULES OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES
title_sort mediators of inflammation in leukocyte lysosomes : ix. elastinolytic activity in granules of human polymorphonuclear leukocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138566/
https://www.ncbi.nlm.nih.gov/pubmed/5303065
work_keys_str_mv AT janoffa mediatorsofinflammationinleukocytelysosomesixelastinolyticactivityingranulesofhumanpolymorphonuclearleukocytes
AT schererj mediatorsofinflammationinleukocytelysosomesixelastinolyticactivityingranulesofhumanpolymorphonuclearleukocytes