CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON

In the presence of specific antigen, lymph node cells from inbred rats with delayed hypersensitivity to tuberculoprotein, bovine gammaglobulin, and egg albumin produced progressive destruction of monolayers of rat embryo fibroblasts in tissue culture, first apparent at 48 hr and maximal at 72 hr. Th...

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Detalles Bibliográficos
Autores principales: Ruddle, Nancy H., Waksman, Byron H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138572/
https://www.ncbi.nlm.nih.gov/pubmed/4176937
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author Ruddle, Nancy H.
Waksman, Byron H.
author_facet Ruddle, Nancy H.
Waksman, Byron H.
author_sort Ruddle, Nancy H.
collection PubMed
description In the presence of specific antigen, lymph node cells from inbred rats with delayed hypersensitivity to tuberculoprotein, bovine gammaglobulin, and egg albumin produced progressive destruction of monolayers of rat embryo fibroblasts in tissue culture, first apparent at 48 hr and maximal at 72 hr. The effect was specific and did not depend on a genetic difference between the lymph node cells and target cells. It required antigen concentrations equal to or greater than 1.25 µg/ml and lymphocyte: target cell ratios of approximately 10 or 20:1. It could be evaluated both by a plaquing technique and by cell enumeration with an electronic particle counter.
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spelling pubmed-21385722008-04-17 CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON Ruddle, Nancy H. Waksman, Byron H. J Exp Med Article In the presence of specific antigen, lymph node cells from inbred rats with delayed hypersensitivity to tuberculoprotein, bovine gammaglobulin, and egg albumin produced progressive destruction of monolayers of rat embryo fibroblasts in tissue culture, first apparent at 48 hr and maximal at 72 hr. The effect was specific and did not depend on a genetic difference between the lymph node cells and target cells. It required antigen concentrations equal to or greater than 1.25 µg/ml and lymphocyte: target cell ratios of approximately 10 or 20:1. It could be evaluated both by a plaquing technique and by cell enumeration with an electronic particle counter. The Rockefeller University Press 1968-11-30 /pmc/articles/PMC2138572/ /pubmed/4176937 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ruddle, Nancy H.
Waksman, Byron H.
CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON
title CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON
title_full CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON
title_fullStr CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON
title_full_unstemmed CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON
title_short CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY : I. CHARACTERIZATION OF THE PHENOMENON
title_sort cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity : i. characterization of the phenomenon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138572/
https://www.ncbi.nlm.nih.gov/pubmed/4176937
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