USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY

Hybrid antibody [F(ab')(2)] with dual specificity for mouse γG and ferritin was prepared from the corresponding rabbit antisera, providing a precise reagent for locating mouse γG on cell surfaces. Viable cells were exposed successively to (a) mouse antibody to a cell surface antigen, (b) the ra...

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Detalles Bibliográficos
Autores principales: Hämmerling, Ulrich, Aoki, Tadao, de Harven, Etienne, Boyse, Edward A., Old, Lloyd J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138585/
https://www.ncbi.nlm.nih.gov/pubmed/4972125
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author Hämmerling, Ulrich
Aoki, Tadao
de Harven, Etienne
Boyse, Edward A.
Old, Lloyd J.
author_facet Hämmerling, Ulrich
Aoki, Tadao
de Harven, Etienne
Boyse, Edward A.
Old, Lloyd J.
author_sort Hämmerling, Ulrich
collection PubMed
description Hybrid antibody [F(ab')(2)] with dual specificity for mouse γG and ferritin was prepared from the corresponding rabbit antisera, providing a precise reagent for locating mouse γG on cell surfaces. Viable cells were exposed successively to (a) mouse antibody to a cell surface antigen, (b) the rabbit hybrid antibody, and (c) ferritin, before preparation for electron microscopy. This method of See PDF for Structure. labeling is sensitive and specific and clearly lends itself to the introduction of visual markers other than ferritin. Other advantages are uniformity of labeling, ease of purification of the reagent, and circumvention of the many drawbacks arising from coupling ferritin to antibody chemically.
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spelling pubmed-21385852008-04-17 USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY Hämmerling, Ulrich Aoki, Tadao de Harven, Etienne Boyse, Edward A. Old, Lloyd J. J Exp Med Article Hybrid antibody [F(ab')(2)] with dual specificity for mouse γG and ferritin was prepared from the corresponding rabbit antisera, providing a precise reagent for locating mouse γG on cell surfaces. Viable cells were exposed successively to (a) mouse antibody to a cell surface antigen, (b) the rabbit hybrid antibody, and (c) ferritin, before preparation for electron microscopy. This method of See PDF for Structure. labeling is sensitive and specific and clearly lends itself to the introduction of visual markers other than ferritin. Other advantages are uniformity of labeling, ease of purification of the reagent, and circumvention of the many drawbacks arising from coupling ferritin to antibody chemically. The Rockefeller University Press 1968-11-30 /pmc/articles/PMC2138585/ /pubmed/4972125 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hämmerling, Ulrich
Aoki, Tadao
de Harven, Etienne
Boyse, Edward A.
Old, Lloyd J.
USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY
title USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY
title_full USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY
title_fullStr USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY
title_full_unstemmed USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY
title_short USE OF HYBRID ANTIBODY WITH ANTI-γG AND ANTI-FERRITIN SPECIFICITIES IN LOCATING CELL SURFACE ANTIGENS BY ELECTRON MICROSCOPY
title_sort use of hybrid antibody with anti-γg and anti-ferritin specificities in locating cell surface antigens by electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138585/
https://www.ncbi.nlm.nih.gov/pubmed/4972125
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