Cargando…

ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE

The numbers of antigen-reactive cells (ARC) responding to a purified protein, the polymer of S. adelaide flagellin, have been assayed in cell populations derived from several lymphoid tissues of mice. The assay, which employs the cell transfer into lethally irradiated mice, indicates that there is a...

Descripción completa

Detalles Bibliográficos
Autores principales: Armstrong, W. D., Diener, E., Shellam, G. R.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138609/
https://www.ncbi.nlm.nih.gov/pubmed/5762050
_version_ 1782143603245580288
author Armstrong, W. D.
Diener, E.
Shellam, G. R.
author_facet Armstrong, W. D.
Diener, E.
Shellam, G. R.
author_sort Armstrong, W. D.
collection PubMed
description The numbers of antigen-reactive cells (ARC) responding to a purified protein, the polymer of S. adelaide flagellin, have been assayed in cell populations derived from several lymphoid tissues of mice. The assay, which employs the cell transfer into lethally irradiated mice, indicates that there is a response of ARC in bone marrow in the absence of thymus cells. This suggests that the immune response to this protein antigen is not thymus dependent. The presence of relatively large numbers of ARC in Peyer's patches argues for their direct participation in the immune response in the adult mouse. The kinetics of ARC and antibody-forming cells in the early primary response employing the transfer system is described. The numbers of ARC declined during the first 2 days of the immune response, but by day 6 had increased to about five times the number in unprimed spleen cells. The rise is believed to be a result of the primary injection of antigen and therefore may be described as memory; however, these experiments have not been able to further elucidate any specific qualities of the "memory cell." Tolerance induction in C(57)BL/Brad mice produced by repeated injections of a cyanogen bromide digest of the antigen is described. The ARC or its precursor is shown to be the site of the lesion of tolerance by direct investigation.
format Text
id pubmed-2138609
institution National Center for Biotechnology Information
language English
publishDate 1969
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21386092008-04-17 ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE Armstrong, W. D. Diener, E. Shellam, G. R. J Exp Med Article The numbers of antigen-reactive cells (ARC) responding to a purified protein, the polymer of S. adelaide flagellin, have been assayed in cell populations derived from several lymphoid tissues of mice. The assay, which employs the cell transfer into lethally irradiated mice, indicates that there is a response of ARC in bone marrow in the absence of thymus cells. This suggests that the immune response to this protein antigen is not thymus dependent. The presence of relatively large numbers of ARC in Peyer's patches argues for their direct participation in the immune response in the adult mouse. The kinetics of ARC and antibody-forming cells in the early primary response employing the transfer system is described. The numbers of ARC declined during the first 2 days of the immune response, but by day 6 had increased to about five times the number in unprimed spleen cells. The rise is believed to be a result of the primary injection of antigen and therefore may be described as memory; however, these experiments have not been able to further elucidate any specific qualities of the "memory cell." Tolerance induction in C(57)BL/Brad mice produced by repeated injections of a cyanogen bromide digest of the antigen is described. The ARC or its precursor is shown to be the site of the lesion of tolerance by direct investigation. The Rockefeller University Press 1969-01-31 /pmc/articles/PMC2138609/ /pubmed/5762050 Text en Copyright © 1969 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Armstrong, W. D.
Diener, E.
Shellam, G. R.
ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE
title ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE
title_full ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE
title_fullStr ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE
title_full_unstemmed ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE
title_short ANTIGEN-REACTIVE CELLS IN NORMAL, IMMUNIZED, AND TOLERANT MICE
title_sort antigen-reactive cells in normal, immunized, and tolerant mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138609/
https://www.ncbi.nlm.nih.gov/pubmed/5762050
work_keys_str_mv AT armstrongwd antigenreactivecellsinnormalimmunizedandtolerantmice
AT dienere antigenreactivecellsinnormalimmunizedandtolerantmice
AT shellamgr antigenreactivecellsinnormalimmunizedandtolerantmice