COLICIN K : VIII. THE IMMUNOLOGICAL PROPERTIES OF MITOMYCIN-INDUCED COLICIN K

Partially purified colicin K (mCol K) has been obtained from cultures of Escherichia coli K235 induced with mitomycin C. Unlike colicin K (Col K) derived from noninduced cultures of E. coli K235, mitomycin-induced colicin K (mCol K) is not associated with the type O-specific antigen of the colicinog...

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Detalles Bibliográficos
Autores principales: Tsao, Shuang-Shine, Goebel, Walther F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138701/
https://www.ncbi.nlm.nih.gov/pubmed/4187527
Descripción
Sumario:Partially purified colicin K (mCol K) has been obtained from cultures of Escherichia coli K235 induced with mitomycin C. Unlike colicin K (Col K) derived from noninduced cultures of E. coli K235, mitomycin-induced colicin K (mCol K) is not associated with the type O-specific antigen of the colicinogenic bacillus. mCol K elicits in rabbits specific antibodies which precipitate and neutralize the homologous bacteriocin. These colicin-specific antibodies are not precipitated by the colicin-O antigen complex derived from noninduced bacteria. Colicin-neutralizing antibodies can be separated by zone electrophoresis into fractions having different electrophoretic mobilities. The antibodies with lower mobility strongly precipitate the homologous antigen mCol K; those with higher mobility neutralize the bacteriocin and form soluble antigen-antibody complexes.

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