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INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS

Thoracic duct cells and spleen cells were tested for their ability to restore the primary antibody response of X-irradiated rats to bovine serum albumin (BSA), sheep red blood cells (SRBC), horse spleen femtin (HSF), and Salmonella typhi flagella. Spleen cells were at least as efficient as thoracic...

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Detalles Bibliográficos
Autor principal: Strober, Samuel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138718/
https://www.ncbi.nlm.nih.gov/pubmed/5343440
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author Strober, Samuel
author_facet Strober, Samuel
author_sort Strober, Samuel
collection PubMed
description Thoracic duct cells and spleen cells were tested for their ability to restore the primary antibody response of X-irradiated rats to bovine serum albumin (BSA), sheep red blood cells (SRBC), horse spleen femtin (HSF), and Salmonella typhi flagella. Spleen cells were at least as efficient as thoracic duct cells in restoring the response to BSA, HSF, and Salmonella typhi flagella. In further experiments thoracic duct cells lacking large dividing lymphocytes were tested for their ability to restore the primary response. Large lymphocytes were eliminated by the in vitro incubation of thoracic duct cells for 24 hr at 37°C or by treatment of thoracic duct cell donors with the mitotic inhibitor vinblastine sulfate 24 hr prior to cannulation of the thoracic duct. Experiments with SRBC show that incubated cells and cells from vinblastine-treated donors are as efficient as normal cells in restoring the primary antibody response. On the other hand, experiments with HSF and Salmonella typhi flagella show that incubated cells and cells from vinblastine-treated donors are about five times less efficient than normal cells in restoring the response. Normal thoracic duct cells were more efficient than incubated cells but less efficient than cells from vinblastine-treated donors in restoring the early response to BSA. The experimental findings indicate that the classes of thoracic duct lymphocytes which initiate the primary antibody response to SRBC differ from the classes which initiate the response to HSF and Salmonella typhi flagella, or BSA.
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spelling pubmed-21387182008-04-17 INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS Strober, Samuel J Exp Med Article Thoracic duct cells and spleen cells were tested for their ability to restore the primary antibody response of X-irradiated rats to bovine serum albumin (BSA), sheep red blood cells (SRBC), horse spleen femtin (HSF), and Salmonella typhi flagella. Spleen cells were at least as efficient as thoracic duct cells in restoring the response to BSA, HSF, and Salmonella typhi flagella. In further experiments thoracic duct cells lacking large dividing lymphocytes were tested for their ability to restore the primary response. Large lymphocytes were eliminated by the in vitro incubation of thoracic duct cells for 24 hr at 37°C or by treatment of thoracic duct cell donors with the mitotic inhibitor vinblastine sulfate 24 hr prior to cannulation of the thoracic duct. Experiments with SRBC show that incubated cells and cells from vinblastine-treated donors are as efficient as normal cells in restoring the primary antibody response. On the other hand, experiments with HSF and Salmonella typhi flagella show that incubated cells and cells from vinblastine-treated donors are about five times less efficient than normal cells in restoring the response. Normal thoracic duct cells were more efficient than incubated cells but less efficient than cells from vinblastine-treated donors in restoring the early response to BSA. The experimental findings indicate that the classes of thoracic duct lymphocytes which initiate the primary antibody response to SRBC differ from the classes which initiate the response to HSF and Salmonella typhi flagella, or BSA. The Rockefeller University Press 1969-10-01 /pmc/articles/PMC2138718/ /pubmed/5343440 Text en Copyright © 1969 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Strober, Samuel
INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS
title INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS
title_full INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS
title_fullStr INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS
title_full_unstemmed INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS
title_short INITIATION OF ANTIBODY RESPONSES BY DIFFERENT CLASSES OF LYMPHOCYTES : I. TYPES OF THORACIC DUCT LYMPHOCYTES INVOLVED IN PRIMARY ANTIBODY RESPONSES OF RATS
title_sort initiation of antibody responses by different classes of lymphocytes : i. types of thoracic duct lymphocytes involved in primary antibody responses of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138718/
https://www.ncbi.nlm.nih.gov/pubmed/5343440
work_keys_str_mv AT strobersamuel initiationofantibodyresponsesbydifferentclassesoflymphocytesitypesofthoracicductlymphocytesinvolvedinprimaryantibodyresponsesofrats