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IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS

Eight preparations of soluble amyloid and degraded amyloid (DAM) were compared immunologically. Unlike amyloid fibrils, six of eight preparations of DAM proved to be relatively strong immunogens. Antisera to DAM reacted weakly or not at all with normal human serum or extracts of normal tissues, but...

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Detalles Bibliográficos
Autores principales: Franklin, Edward C., Pras, Mordechai
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138729/
https://www.ncbi.nlm.nih.gov/pubmed/5343434
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author Franklin, Edward C.
Pras, Mordechai
author_facet Franklin, Edward C.
Pras, Mordechai
author_sort Franklin, Edward C.
collection PubMed
description Eight preparations of soluble amyloid and degraded amyloid (DAM) were compared immunologically. Unlike amyloid fibrils, six of eight preparations of DAM proved to be relatively strong immunogens. Antisera to DAM reacted weakly or not at all with normal human serum or extracts of normal tissues, but were specifically reactive with amyloid fibrils or DAM. Comparative studies of DAM'S from eight different subjects showed some degree of cross-reactivity among them, yet demonstrated that they were not identical. Similar conclusions were obtained by quantitative precipitin and complement fixation analyses. Comparison of the amyloid fibrils with the homologous DAM by complement fixation and absorption studies demonstrated the existence in DAM of antigenic determinants that were lacking or inaccessible in the native fibrils. A search for amyloid precursors and antibodies to amyloid in the sera of 12 patients proved unsuccessful.
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spelling pubmed-21387292008-04-17 IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS Franklin, Edward C. Pras, Mordechai J Exp Med Article Eight preparations of soluble amyloid and degraded amyloid (DAM) were compared immunologically. Unlike amyloid fibrils, six of eight preparations of DAM proved to be relatively strong immunogens. Antisera to DAM reacted weakly or not at all with normal human serum or extracts of normal tissues, but were specifically reactive with amyloid fibrils or DAM. Comparative studies of DAM'S from eight different subjects showed some degree of cross-reactivity among them, yet demonstrated that they were not identical. Similar conclusions were obtained by quantitative precipitin and complement fixation analyses. Comparison of the amyloid fibrils with the homologous DAM by complement fixation and absorption studies demonstrated the existence in DAM of antigenic determinants that were lacking or inaccessible in the native fibrils. A search for amyloid precursors and antibodies to amyloid in the sera of 12 patients proved unsuccessful. The Rockefeller University Press 1969-10-01 /pmc/articles/PMC2138729/ /pubmed/5343434 Text en Copyright © 1969 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Franklin, Edward C.
Pras, Mordechai
IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS
title IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS
title_full IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS
title_fullStr IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS
title_full_unstemmed IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS
title_short IMMUNOLOGIC STUDIES OF WATER-SOLUBLE HUMAN AMYLOID FIBRILS : COMPARATIVE STUDIES OF EIGHT AMYLOID PREPARATIONS
title_sort immunologic studies of water-soluble human amyloid fibrils : comparative studies of eight amyloid preparations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138729/
https://www.ncbi.nlm.nih.gov/pubmed/5343434
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