THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM

Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The...

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Detalles Bibliográficos
Autores principales: Gordon, James H., Dubos, René
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138733/
https://www.ncbi.nlm.nih.gov/pubmed/4927597
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author Gordon, James H.
Dubos, René
author_facet Gordon, James H.
Dubos, René
author_sort Gordon, James H.
collection PubMed
description Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The commonest morphologic types of bacteria in the mouse cecum were tapered rods. These characteristically gave "speckled" colonies on agar plates. Those identified were species of the genera Fusobacterium, Eubacterium, and Clostridium. Several of the morphologic types seen with phase microscopy still could not be cultivated. The implications of these findings for the study of the intestinal flora are discussed.
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spelling pubmed-21387332008-04-17 THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM Gordon, James H. Dubos, René J Exp Med Article Refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of SPF mice. The commonest morphologic types of bacteria in the mouse cecum were tapered rods. These characteristically gave "speckled" colonies on agar plates. Those identified were species of the genera Fusobacterium, Eubacterium, and Clostridium. Several of the morphologic types seen with phase microscopy still could not be cultivated. The implications of these findings for the study of the intestinal flora are discussed. The Rockefeller University Press 1970-08-01 /pmc/articles/PMC2138733/ /pubmed/4927597 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gordon, James H.
Dubos, René
THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM
title THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM
title_full THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM
title_fullStr THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM
title_full_unstemmed THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM
title_short THE ANAEROBIC BACTERIAL FLORA OF THE MOUSE CECUM
title_sort anaerobic bacterial flora of the mouse cecum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138733/
https://www.ncbi.nlm.nih.gov/pubmed/4927597
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