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CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION

Preimmunization of either guinea pigs or rabbits to bovine gamma globulin (BGG) prepares the animals for markedly enhanced antibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). This phenomenon is observed both in the primary anti-DNP antibody response to DNP-BGG and in the secondary anti-DNP antibo...

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Autores principales: Katz, David H., Paul, William E., Goidl, Edmond A., Benacerraf, Baruj
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138738/
https://www.ncbi.nlm.nih.gov/pubmed/4101344
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author Katz, David H.
Paul, William E.
Goidl, Edmond A.
Benacerraf, Baruj
author_facet Katz, David H.
Paul, William E.
Goidl, Edmond A.
Benacerraf, Baruj
author_sort Katz, David H.
collection PubMed
description Preimmunization of either guinea pigs or rabbits to bovine gamma globulin (BGG) prepares the animals for markedly enhanced antibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). This phenomenon is observed both in the primary anti-DNP antibody response to DNP-BGG and in the secondary anti-DNP antibody response to DNP-BGG in animals primed with DNP-ovalbumin (DNP-OVA). The BGG preimmunization is most effective if the antigen is administered as a complete Freund's adjuvant emulsion; in rabbits, a dose of 1 µg of BGG is more effective than a dose of 50 µg, whereas the reverse is true in guinea pigs. Transfusion of homologous anti-BGG sera fails to replace active immunization with BGG in the preparation of animals for these enhanced anti-DNP antibody responses. Both the immunoglobulin class and the average association constant for ε-DNP-L-lysine of the anti-DNP antibody produced in these enhanced responses is determined by the mode and time of immunization with haptenic conjugates and is not appreciably influenced by the nature of the carrier preimmunization. These studies indicate that the carrier specificity of hapten-specific anamnestic antibody responses is largely due to the interaction of two independent cell associated recognition units, one specialized for carrier and the other specific for haptenic determinants.
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spelling pubmed-21387382008-04-17 CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION Katz, David H. Paul, William E. Goidl, Edmond A. Benacerraf, Baruj J Exp Med Article Preimmunization of either guinea pigs or rabbits to bovine gamma globulin (BGG) prepares the animals for markedly enhanced antibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). This phenomenon is observed both in the primary anti-DNP antibody response to DNP-BGG and in the secondary anti-DNP antibody response to DNP-BGG in animals primed with DNP-ovalbumin (DNP-OVA). The BGG preimmunization is most effective if the antigen is administered as a complete Freund's adjuvant emulsion; in rabbits, a dose of 1 µg of BGG is more effective than a dose of 50 µg, whereas the reverse is true in guinea pigs. Transfusion of homologous anti-BGG sera fails to replace active immunization with BGG in the preparation of animals for these enhanced anti-DNP antibody responses. Both the immunoglobulin class and the average association constant for ε-DNP-L-lysine of the anti-DNP antibody produced in these enhanced responses is determined by the mode and time of immunization with haptenic conjugates and is not appreciably influenced by the nature of the carrier preimmunization. These studies indicate that the carrier specificity of hapten-specific anamnestic antibody responses is largely due to the interaction of two independent cell associated recognition units, one specialized for carrier and the other specific for haptenic determinants. The Rockefeller University Press 1970-08-01 /pmc/articles/PMC2138738/ /pubmed/4101344 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Katz, David H.
Paul, William E.
Goidl, Edmond A.
Benacerraf, Baruj
CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION
title CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION
title_full CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION
title_fullStr CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION
title_full_unstemmed CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION
title_short CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION
title_sort carrier function in anti-hapten immune responses : i. enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138738/
https://www.ncbi.nlm.nih.gov/pubmed/4101344
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