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QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES

Idiotypic antibodies were investigated quantitatively by a method of indirect precipitation, which utilizes labeled F(ab')(2) fragments of specifically purified antibenzoate antibody from the donor, anti-antibody, and an antiglobulin reagent. The contribution of allotypic and hidden determinant...

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Detalles Bibliográficos
Autores principales: Hopper, John E., MacDonald, A. Bruce, Nisonoff, Alfred
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138758/
https://www.ncbi.nlm.nih.gov/pubmed/5308065
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author Hopper, John E.
MacDonald, A. Bruce
Nisonoff, Alfred
author_facet Hopper, John E.
MacDonald, A. Bruce
Nisonoff, Alfred
author_sort Hopper, John E.
collection PubMed
description Idiotypic antibodies were investigated quantitatively by a method of indirect precipitation, which utilizes labeled F(ab')(2) fragments of specifically purified antibenzoate antibody from the donor, anti-antibody, and an antiglobulin reagent. The contribution of allotypic and hidden determinants to these reactions was excluded. Greater fractions of an idiotypic antibody population are precipitated by this method, as compared to direct precipitation, and in two instances large proportions of idiotypic antibodies were detected in populations which failed to form precipitates by double diffusion in agar gel. The greater sensitivity of the indirect method was attributed to its capacity to detect molecules bearing a small number of antigenic determinants. Extensive studies of cross-reactions, carried out by an inhibition technique, failed to reveal any strong reactions of anti-idiotypic antibodies with heterologous antibenzoate antibody preparations, heterologous sera, or IgG, although a few weak cross-reactions were noted. One definite cross-reaction was observed by a direct binding measurement with heterologous antiserum. Antisera prepared in more than one recipient against a single donor preparation reacted with identical or overlapping subpopulations of the donor molecules. Instances in which two recipient antisera reacted with different proportions of the molecules of a single donor provided evidence for the existence of more than one idiotypic antibody population in the antibenzoate antibody of an individual rabbit.
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spelling pubmed-21387582008-04-17 QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES Hopper, John E. MacDonald, A. Bruce Nisonoff, Alfred J Exp Med Article Idiotypic antibodies were investigated quantitatively by a method of indirect precipitation, which utilizes labeled F(ab')(2) fragments of specifically purified antibenzoate antibody from the donor, anti-antibody, and an antiglobulin reagent. The contribution of allotypic and hidden determinants to these reactions was excluded. Greater fractions of an idiotypic antibody population are precipitated by this method, as compared to direct precipitation, and in two instances large proportions of idiotypic antibodies were detected in populations which failed to form precipitates by double diffusion in agar gel. The greater sensitivity of the indirect method was attributed to its capacity to detect molecules bearing a small number of antigenic determinants. Extensive studies of cross-reactions, carried out by an inhibition technique, failed to reveal any strong reactions of anti-idiotypic antibodies with heterologous antibenzoate antibody preparations, heterologous sera, or IgG, although a few weak cross-reactions were noted. One definite cross-reaction was observed by a direct binding measurement with heterologous antiserum. Antisera prepared in more than one recipient against a single donor preparation reacted with identical or overlapping subpopulations of the donor molecules. Instances in which two recipient antisera reacted with different proportions of the molecules of a single donor provided evidence for the existence of more than one idiotypic antibody population in the antibenzoate antibody of an individual rabbit. The Rockefeller University Press 1970-01-01 /pmc/articles/PMC2138758/ /pubmed/5308065 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hopper, John E.
MacDonald, A. Bruce
Nisonoff, Alfred
QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES
title QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES
title_full QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES
title_fullStr QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES
title_full_unstemmed QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES
title_short QUANTITATIVE INVESTIGATIONS OF IDIOTYPIC ANTIBODIES : II. NONPRECIPITATING ANTIBODIES
title_sort quantitative investigations of idiotypic antibodies : ii. nonprecipitating antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138758/
https://www.ncbi.nlm.nih.gov/pubmed/5308065
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