REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7

This paper describes the characteristics of the indicator factor (I) which takes part in reactive hemolysis and its identification as the seventh component of complement. I was shown to be a beta globulin with a sediment coefficient of 5.7S and a molecular weight of about 140,000. Experiments on the...

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Detalles Bibliográficos
Autores principales: Thompson, R. A., Lachmann, P. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138783/
https://www.ncbi.nlm.nih.gov/pubmed/4193934
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author Thompson, R. A.
Lachmann, P. J.
author_facet Thompson, R. A.
Lachmann, P. J.
author_sort Thompson, R. A.
collection PubMed
description This paper describes the characteristics of the indicator factor (I) which takes part in reactive hemolysis and its identification as the seventh component of complement. I was shown to be a beta globulin with a sediment coefficient of 5.7S and a molecular weight of about 140,000. Experiments on the depletion of I activity with anti-I antiserum or with activated R euglobulin showed that I was a late acting complement component necessary for the lysis of cells after the EAC142 stage. Complement component analysis of purified I fractions excluded all known components except C7. The physicochemical characteristics of I are compatible with published data on C7. The method of quantitation described represents a convenient method of testing for C7.
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spelling pubmed-21387832008-04-17 REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7 Thompson, R. A. Lachmann, P. J. J Exp Med Article This paper describes the characteristics of the indicator factor (I) which takes part in reactive hemolysis and its identification as the seventh component of complement. I was shown to be a beta globulin with a sediment coefficient of 5.7S and a molecular weight of about 140,000. Experiments on the depletion of I activity with anti-I antiserum or with activated R euglobulin showed that I was a late acting complement component necessary for the lysis of cells after the EAC142 stage. Complement component analysis of purified I fractions excluded all known components except C7. The physicochemical characteristics of I are compatible with published data on C7. The method of quantitation described represents a convenient method of testing for C7. The Rockefeller University Press 1970-03-31 /pmc/articles/PMC2138783/ /pubmed/4193934 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Thompson, R. A.
Lachmann, P. J.
REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7
title REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7
title_full REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7
title_fullStr REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7
title_full_unstemmed REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7
title_short REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7
title_sort reactive lysis: the complement-mediated lysis of unsensitized cells : i. the characterization of the indicator factor and its identification as c7
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138783/
https://www.ncbi.nlm.nih.gov/pubmed/4193934
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