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THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN

The interaction in vitro between human granulocytes and meningococci in the presence of sera from volunteers immunized by Gotschlich et al. with purified group A and group C meningococcal polysaccharides was studied. Phagocytosis of meningococci did not occur in the presence of preimmunization sera....

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Autor principal: Roberts, Richard B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138825/
https://www.ncbi.nlm.nih.gov/pubmed/4189835
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author Roberts, Richard B.
author_facet Roberts, Richard B.
author_sort Roberts, Richard B.
collection PubMed
description The interaction in vitro between human granulocytes and meningococci in the presence of sera from volunteers immunized by Gotschlich et al. with purified group A and group C meningococcal polysaccharides was studied. Phagocytosis of meningococci did not occur in the presence of preimmunization sera. In all volunteers tested, group-specific opsonins were detected in groups A and C polysaccharide antisera. Opsonic activity appeared within 1 wk after immunization and persisted for at least 14 months. Titers of opsonic activity ranged from 1:20 to 1:320; highest titers were noted in 2–4 wk antisera. Meningococcal opsonins were detected in both 19S and 7S immunoglobulins. Opsonic activity in low-titer antisera depended on heat-labile factors present in both normal and immune sera, whereas phagocytosis was observed in the presence of heat-inactivated high-titer antisera. Phagocytosis of group A meningococci in the presence of certain group A polysaccharide antisera was inhibited by N-acetyl mannosamine, but not by mannose, mannosamine, N-acetyl glucosamine, N-acetyl galactosamine, or N-acetyl neuraminic acid. Absorption studies with sera from patients with natural meningococcal infections revealed that these polysaccharides are the major antiphagocytic determinants for group A and group C meningococci. These studies are consistent with previous reports suggesting that immunization with group A and group C polysaccharides may well provide group-specific protection against meningococcal infections.
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spelling pubmed-21388252008-04-17 THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN Roberts, Richard B. J Exp Med Article The interaction in vitro between human granulocytes and meningococci in the presence of sera from volunteers immunized by Gotschlich et al. with purified group A and group C meningococcal polysaccharides was studied. Phagocytosis of meningococci did not occur in the presence of preimmunization sera. In all volunteers tested, group-specific opsonins were detected in groups A and C polysaccharide antisera. Opsonic activity appeared within 1 wk after immunization and persisted for at least 14 months. Titers of opsonic activity ranged from 1:20 to 1:320; highest titers were noted in 2–4 wk antisera. Meningococcal opsonins were detected in both 19S and 7S immunoglobulins. Opsonic activity in low-titer antisera depended on heat-labile factors present in both normal and immune sera, whereas phagocytosis was observed in the presence of heat-inactivated high-titer antisera. Phagocytosis of group A meningococci in the presence of certain group A polysaccharide antisera was inhibited by N-acetyl mannosamine, but not by mannose, mannosamine, N-acetyl glucosamine, N-acetyl galactosamine, or N-acetyl neuraminic acid. Absorption studies with sera from patients with natural meningococcal infections revealed that these polysaccharides are the major antiphagocytic determinants for group A and group C meningococci. These studies are consistent with previous reports suggesting that immunization with group A and group C polysaccharides may well provide group-specific protection against meningococcal infections. The Rockefeller University Press 1970-02-28 /pmc/articles/PMC2138825/ /pubmed/4189835 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Roberts, Richard B.
THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN
title THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN
title_full THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN
title_fullStr THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN
title_full_unstemmed THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN
title_short THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN
title_sort relationship between group a and group c meningococcal polysaccharides and serum opsonins in man
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138825/
https://www.ncbi.nlm.nih.gov/pubmed/4189835
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