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IN VITRO STIMULATION OF ANTIBODY FORMATION BY PERITONEAL CELLS : I. PLAQUE TECHNIQUE OF HIGH SENSITIVITY ENABLING ACCESS TO THE CELLS
An improved method for the short-term culture of mouse peritoneal cells in a medium containing carboxymethylcellulose (CMC), sheep erythrocytes (SRBC), and guinea pig complement is described. It involves preparation of microcultures, of thickness 12–15 µ and volume 3.6 µl, under paraffin oil. With s...
Autores principales: | Nossal, G. J. V., Bussard, A. E., Lewis, H., Mazie, J. C. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1970
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138832/ https://www.ncbi.nlm.nih.gov/pubmed/4910143 |
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