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HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY
Antigen-mediated stimulation of thymidine incorporation was demonstrated in lymph node cells from guinea pigs immunized with 100 µg human serum albumin in either Freund's incomplete or Freund's complete adjuvant. Animals receiving HSA in IFA exhibited both cutaneous basophil (Jones-Mote) h...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138899/ https://www.ncbi.nlm.nih.gov/pubmed/5316262 |
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author | Bast, Robert C. Simpson, Blanche A. Dvorak, Harold F. |
author_facet | Bast, Robert C. Simpson, Blanche A. Dvorak, Harold F. |
author_sort | Bast, Robert C. |
collection | PubMed |
description | Antigen-mediated stimulation of thymidine incorporation was demonstrated in lymph node cells from guinea pigs immunized with 100 µg human serum albumin in either Freund's incomplete or Freund's complete adjuvant. Animals receiving HSA in IFA exhibited both cutaneous basophil (Jones-Mote) hypersensitivity and lymphocyte stimulation at 1, but not at 6 wk after immunization. Significant stimulation required ≥ 10 µg HSA/ml of culture. Sensitization with HSA in CFA produced delayed hypersensitivity and permitted lymphocyte stimulation at both 1 and 6 wk. Stimulation was observed with as little as 0.1 µg HSA/ml at the later interval. Administration of 5 mg HSA intravenously at the time of sensitization with 100 µg HSA in IFA reduced but did not eliminate both CBH and lymphocyte stimulation at 1 wk. Antigen-specific inhibition of macrophage migration could be demonstrated with exudates from animals immunized with HSA in CFA, but not with HSA in IFA at 3 wk after sensitization. HSA was cleared from depots of CFA and IFA at similar rates, but significantly more antigen appeared in the plasma and subsequently in the draining lymph nodes following administration in IFA. Conversely, accumulated antigen disappeared more rapidly following CFA immunization. |
format | Text |
id | pubmed-2138899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21388992008-04-17 HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY Bast, Robert C. Simpson, Blanche A. Dvorak, Harold F. J Exp Med Article Antigen-mediated stimulation of thymidine incorporation was demonstrated in lymph node cells from guinea pigs immunized with 100 µg human serum albumin in either Freund's incomplete or Freund's complete adjuvant. Animals receiving HSA in IFA exhibited both cutaneous basophil (Jones-Mote) hypersensitivity and lymphocyte stimulation at 1, but not at 6 wk after immunization. Significant stimulation required ≥ 10 µg HSA/ml of culture. Sensitization with HSA in CFA produced delayed hypersensitivity and permitted lymphocyte stimulation at both 1 and 6 wk. Stimulation was observed with as little as 0.1 µg HSA/ml at the later interval. Administration of 5 mg HSA intravenously at the time of sensitization with 100 µg HSA in IFA reduced but did not eliminate both CBH and lymphocyte stimulation at 1 wk. Antigen-specific inhibition of macrophage migration could be demonstrated with exudates from animals immunized with HSA in CFA, but not with HSA in IFA at 3 wk after sensitization. HSA was cleared from depots of CFA and IFA at similar rates, but significantly more antigen appeared in the plasma and subsequently in the draining lymph nodes following administration in IFA. Conversely, accumulated antigen disappeared more rapidly following CFA immunization. The Rockefeller University Press 1971-01-31 /pmc/articles/PMC2138899/ /pubmed/5316262 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Bast, Robert C. Simpson, Blanche A. Dvorak, Harold F. HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY |
title | HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY |
title_full | HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY |
title_fullStr | HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY |
title_full_unstemmed | HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY |
title_short | HETEROGENEITY OF THE CELLULAR IMMUNE RESPONSE : II. THE ROLE OF ADJUVANT. LYMPHOCYTE STIMULATION IN CUTANEOUS BASOPHIL HYPERSENSITIVITY |
title_sort | heterogeneity of the cellular immune response : ii. the role of adjuvant. lymphocyte stimulation in cutaneous basophil hypersensitivity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138899/ https://www.ncbi.nlm.nih.gov/pubmed/5316262 |
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