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SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS
Bone marrow or lymph node cells from 10 patients whose sera contained large amounts of monoclonal IgM proteins were incubated with radioactive amino acids in short-term tissue culture. Samples of soluble cytoplasmic extracts and secreted material were examined by immunologic precipitation with speci...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138919/ https://www.ncbi.nlm.nih.gov/pubmed/4995064 |
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author | Buxbaum, J. Zolla, Susan Scharff, M. D. Franklin, E. C. |
author_facet | Buxbaum, J. Zolla, Susan Scharff, M. D. Franklin, E. C. |
author_sort | Buxbaum, J. |
collection | PubMed |
description | Bone marrow or lymph node cells from 10 patients whose sera contained large amounts of monoclonal IgM proteins were incubated with radioactive amino acids in short-term tissue culture. Samples of soluble cytoplasmic extracts and secreted material were examined by immunologic precipitation with specific antisera, acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and sucrose gradient centrifugation. In all samples studied, 8S IgM was the major intracellular precursor of the fully assembled 19S protein. Cells obtained from some patients contained little or no fully assembled 19S protein intracellularly; however, cells from most patients seemed to accumulate fully assembled 19S molecules intracellularly before secretion. Secreted material from both groups contained large amounts of 19S IgM. The differentiation between accumulating and nonaccumulating cells did not correlate with heavy or light chain antigenic type. Synthesis and assembly appeared to be identical in cells obtained from different anatomic sites in the same patient Studies carried out in one patient before and after therapy revealed no qualitative differences in the pathway of assembly and reflected only a decrease in the total number of immunoglobulin-producing cells. |
format | Text |
id | pubmed-2138919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21389192008-04-17 SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS Buxbaum, J. Zolla, Susan Scharff, M. D. Franklin, E. C. J Exp Med Article Bone marrow or lymph node cells from 10 patients whose sera contained large amounts of monoclonal IgM proteins were incubated with radioactive amino acids in short-term tissue culture. Samples of soluble cytoplasmic extracts and secreted material were examined by immunologic precipitation with specific antisera, acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and sucrose gradient centrifugation. In all samples studied, 8S IgM was the major intracellular precursor of the fully assembled 19S protein. Cells obtained from some patients contained little or no fully assembled 19S protein intracellularly; however, cells from most patients seemed to accumulate fully assembled 19S molecules intracellularly before secretion. Secreted material from both groups contained large amounts of 19S IgM. The differentiation between accumulating and nonaccumulating cells did not correlate with heavy or light chain antigenic type. Synthesis and assembly appeared to be identical in cells obtained from different anatomic sites in the same patient Studies carried out in one patient before and after therapy revealed no qualitative differences in the pathway of assembly and reflected only a decrease in the total number of immunoglobulin-producing cells. The Rockefeller University Press 1971-05-01 /pmc/articles/PMC2138919/ /pubmed/4995064 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Buxbaum, J. Zolla, Susan Scharff, M. D. Franklin, E. C. SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS |
title | SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS |
title_full | SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS |
title_fullStr | SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS |
title_full_unstemmed | SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS |
title_short | SYNTHESIS AND ASSEMBLY OF IMMUNOGLOBULINS BY MALIGNANT HUMAN PLASMACYTES AND LYMPHOCYTES : II. HETEROGENEITY OF ASSEMBLY IN CELLS PRODUCING IGM PROTEINS |
title_sort | synthesis and assembly of immunoglobulins by malignant human plasmacytes and lymphocytes : ii. heterogeneity of assembly in cells producing igm proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138919/ https://www.ncbi.nlm.nih.gov/pubmed/4995064 |
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