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CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY
An in vitro model of cellular immunity in the guinea pig was established. Animals were immunized with tubercle bacilli, bovine gamma globulin, or picrylated human serum albumin in complete Freund's adjuvant. Oil-induced peritoneal exudates from immune and control animals were cultured overnight...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138929/ https://www.ncbi.nlm.nih.gov/pubmed/4102688 |
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author | Simon, Harvey B. Sheagren, John N. |
author_facet | Simon, Harvey B. Sheagren, John N. |
author_sort | Simon, Harvey B. |
collection | PubMed |
description | An in vitro model of cellular immunity in the guinea pig was established. Animals were immunized with tubercle bacilli, bovine gamma globulin, or picrylated human serum albumin in complete Freund's adjuvant. Oil-induced peritoneal exudates from immune and control animals were cultured overnight with and without specific antigen. The cultures were washed and the macrophage monolayers were infected with Listeria monocytogenes. At intervals the monolayers were lysed and the numbers of viable intracellular bacteria were quantitated by pour plate cultures. Random monolayers were also evaluated in sequence by visually counting the intracellular bacteria on Gram-stained plates. Both methods demonstrated that the macrophages from immune animals had markedly enhanced listericidal activity when the peritoneal exudates were cultured with antigen before infection. Macrophage migration inhibition was also demonstrated under these conditions. The experiments reported here describe an in vitro model of cellular immunity which will allow separation and recombination of cell types and direct assay of cell products in efforts to elucidate further the mechanisms of the immunologically mediated enhancement of macrophage bactericidal capacity. |
format | Text |
id | pubmed-2138929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21389292008-04-17 CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY Simon, Harvey B. Sheagren, John N. J Exp Med Article An in vitro model of cellular immunity in the guinea pig was established. Animals were immunized with tubercle bacilli, bovine gamma globulin, or picrylated human serum albumin in complete Freund's adjuvant. Oil-induced peritoneal exudates from immune and control animals were cultured overnight with and without specific antigen. The cultures were washed and the macrophage monolayers were infected with Listeria monocytogenes. At intervals the monolayers were lysed and the numbers of viable intracellular bacteria were quantitated by pour plate cultures. Random monolayers were also evaluated in sequence by visually counting the intracellular bacteria on Gram-stained plates. Both methods demonstrated that the macrophages from immune animals had markedly enhanced listericidal activity when the peritoneal exudates were cultured with antigen before infection. Macrophage migration inhibition was also demonstrated under these conditions. The experiments reported here describe an in vitro model of cellular immunity which will allow separation and recombination of cell types and direct assay of cell products in efforts to elucidate further the mechanisms of the immunologically mediated enhancement of macrophage bactericidal capacity. The Rockefeller University Press 1971-06-01 /pmc/articles/PMC2138929/ /pubmed/4102688 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Simon, Harvey B. Sheagren, John N. CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY |
title | CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY |
title_full | CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY |
title_fullStr | CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY |
title_full_unstemmed | CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY |
title_short | CELLULAR IMMUNITY IN VITRO : I. IMMUNOLOGICALLY MEDIATED ENHANCEMENT OF MACROPHAGE BACTERICIDAL CAPACITY |
title_sort | cellular immunity in vitro : i. immunologically mediated enhancement of macrophage bactericidal capacity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138929/ https://www.ncbi.nlm.nih.gov/pubmed/4102688 |
work_keys_str_mv | AT simonharveyb cellularimmunityinvitroiimmunologicallymediatedenhancementofmacrophagebactericidalcapacity AT sheagrenjohnn cellularimmunityinvitroiimmunologicallymediatedenhancementofmacrophagebactericidalcapacity |