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IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS
Cells from an established line of Burkitt's lymphoma (Daudi) and a mouse myeloma (P(3)K) were pulse-labeled in vitro with (3)H-leucine, and immunoglobulin was immunologically precipitated from cell lysates and secretions. In contrast to P(3)K cells, Daudi cells synthesize a small amount of Ig w...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138957/ https://www.ncbi.nlm.nih.gov/pubmed/5547061 |
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author | Sherr, Charles J. Uhr, Jonathan W. |
author_facet | Sherr, Charles J. Uhr, Jonathan W. |
author_sort | Sherr, Charles J. |
collection | PubMed |
description | Cells from an established line of Burkitt's lymphoma (Daudi) and a mouse myeloma (P(3)K) were pulse-labeled in vitro with (3)H-leucine, and immunoglobulin was immunologically precipitated from cell lysates and secretions. In contrast to P(3)K cells, Daudi cells synthesize a small amount of Ig which is not secreted. Subcellular fractionation experiments indicated that Ig of Daudi cells is synthesized on membrane-bound polyribosomes and enters the cisternae of the microsomes. Ig in the microsomes could be labeled with either (3)H-galactose or (3)H-fucose suggesting that transport proceeds to the Golgi complex. Additional evidence indicates that Ig molecules are transported to the plasma membrane but are not cleaved from the cell surface. These results together with other studies of Burkitt lymphoma cells suggest that the Daudi line may represent a clone of neoplastic cells derived from normal lymphocytes which synthesize but do not secrete Ig. Similarities between lymphoma cells and antigen-binding cells are discussed. |
format | Text |
id | pubmed-2138957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21389572008-04-17 IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS Sherr, Charles J. Uhr, Jonathan W. J Exp Med Article Cells from an established line of Burkitt's lymphoma (Daudi) and a mouse myeloma (P(3)K) were pulse-labeled in vitro with (3)H-leucine, and immunoglobulin was immunologically precipitated from cell lysates and secretions. In contrast to P(3)K cells, Daudi cells synthesize a small amount of Ig which is not secreted. Subcellular fractionation experiments indicated that Ig of Daudi cells is synthesized on membrane-bound polyribosomes and enters the cisternae of the microsomes. Ig in the microsomes could be labeled with either (3)H-galactose or (3)H-fucose suggesting that transport proceeds to the Golgi complex. Additional evidence indicates that Ig molecules are transported to the plasma membrane but are not cleaved from the cell surface. These results together with other studies of Burkitt lymphoma cells suggest that the Daudi line may represent a clone of neoplastic cells derived from normal lymphocytes which synthesize but do not secrete Ig. Similarities between lymphoma cells and antigen-binding cells are discussed. The Rockefeller University Press 1971-03-31 /pmc/articles/PMC2138957/ /pubmed/5547061 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Sherr, Charles J. Uhr, Jonathan W. IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS |
title | IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS |
title_full | IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS |
title_fullStr | IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS |
title_full_unstemmed | IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS |
title_short | IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS |
title_sort | immunoglobulin synthesis and secretion : vi. synthesis and intracellular transport of immunoglobulin in nonsecretory lymphoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138957/ https://www.ncbi.nlm.nih.gov/pubmed/5547061 |
work_keys_str_mv | AT sherrcharlesj immunoglobulinsynthesisandsecretionvisynthesisandintracellulartransportofimmunoglobulininnonsecretorylymphomacells AT uhrjonathanw immunoglobulinsynthesisandsecretionvisynthesisandintracellulartransportofimmunoglobulininnonsecretorylymphomacells |