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SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG

The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic...

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Detalles Bibliográficos
Autores principales: Baumal, Reuben, Potter, Michael, Scharff, Matthew D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139014/
https://www.ncbi.nlm.nih.gov/pubmed/5112205
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author Baumal, Reuben
Potter, Michael
Scharff, Matthew D.
author_facet Baumal, Reuben
Potter, Michael
Scharff, Matthew D.
author_sort Baumal, Reuben
collection PubMed
description The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic proteins made during a 15 min period. H(2) and H(2)L were the major precursors of IgG(2a) and IgG(1) but, in all of the tumors, HL was also an intermediate. In contrast, HL was a major precursor of IgG(2b). Most of the noncovalent and covalent assembly of IgG occurred after release of the newly synthesized H and L chains from the polyribosomes and assembly was not completed until 10 min or more after the synthesis of the polypeptide chains.
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spelling pubmed-21390142008-04-17 SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG Baumal, Reuben Potter, Michael Scharff, Matthew D. J Exp Med Article The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic proteins made during a 15 min period. H(2) and H(2)L were the major precursors of IgG(2a) and IgG(1) but, in all of the tumors, HL was also an intermediate. In contrast, HL was a major precursor of IgG(2b). Most of the noncovalent and covalent assembly of IgG occurred after release of the newly synthesized H and L chains from the polyribosomes and assembly was not completed until 10 min or more after the synthesis of the polypeptide chains. The Rockefeller University Press 1971-10-31 /pmc/articles/PMC2139014/ /pubmed/5112205 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Baumal, Reuben
Potter, Michael
Scharff, Matthew D.
SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
title SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
title_full SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
title_fullStr SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
title_full_unstemmed SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
title_short SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
title_sort synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells : iii. assembly of the three subclasses of igg
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139014/
https://www.ncbi.nlm.nih.gov/pubmed/5112205
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