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SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG
The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139014/ https://www.ncbi.nlm.nih.gov/pubmed/5112205 |
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author | Baumal, Reuben Potter, Michael Scharff, Matthew D. |
author_facet | Baumal, Reuben Potter, Michael Scharff, Matthew D. |
author_sort | Baumal, Reuben |
collection | PubMed |
description | The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic proteins made during a 15 min period. H(2) and H(2)L were the major precursors of IgG(2a) and IgG(1) but, in all of the tumors, HL was also an intermediate. In contrast, HL was a major precursor of IgG(2b). Most of the noncovalent and covalent assembly of IgG occurred after release of the newly synthesized H and L chains from the polyribosomes and assembly was not completed until 10 min or more after the synthesis of the polypeptide chains. |
format | Text |
id | pubmed-2139014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21390142008-04-17 SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG Baumal, Reuben Potter, Michael Scharff, Matthew D. J Exp Med Article The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic proteins made during a 15 min period. H(2) and H(2)L were the major precursors of IgG(2a) and IgG(1) but, in all of the tumors, HL was also an intermediate. In contrast, HL was a major precursor of IgG(2b). Most of the noncovalent and covalent assembly of IgG occurred after release of the newly synthesized H and L chains from the polyribosomes and assembly was not completed until 10 min or more after the synthesis of the polypeptide chains. The Rockefeller University Press 1971-10-31 /pmc/articles/PMC2139014/ /pubmed/5112205 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Baumal, Reuben Potter, Michael Scharff, Matthew D. SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG |
title | SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG |
title_full | SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG |
title_fullStr | SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG |
title_full_unstemmed | SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG |
title_short | SYNTHESIS, ASSEMBLY, AND SECRETION OF GAMMA GLOBULIN BY MOUSE MYELOMA CELLS : III. ASSEMBLY OF THE THREE SUBCLASSES OF IGG |
title_sort | synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells : iii. assembly of the three subclasses of igg |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139014/ https://www.ncbi.nlm.nih.gov/pubmed/5112205 |
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