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BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES

The importance of C5 in the generation of complement (C)-dependent chemotactic activity in vitro is well recognized. However, the actual role C5 may play in the accumulation of polymorphonuclear leukocytes (PMN) at inflammatory sites in vivo has not been established. Injection of glycogen or endotox...

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Detalles Bibliográficos
Autores principales: Snyderman, Ralph, Phillips, Jean K., Mergenhagen, Stephan E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139022/
https://www.ncbi.nlm.nih.gov/pubmed/5112201
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author Snyderman, Ralph
Phillips, Jean K.
Mergenhagen, Stephan E.
author_facet Snyderman, Ralph
Phillips, Jean K.
Mergenhagen, Stephan E.
author_sort Snyderman, Ralph
collection PubMed
description The importance of C5 in the generation of complement (C)-dependent chemotactic activity in vitro is well recognized. However, the actual role C5 may play in the accumulation of polymorphonuclear leukocytes (PMN) at inflammatory sites in vivo has not been established. Injection of glycogen or endotoxin into the peritoneal cavities of guinea pigs resulted, shortly thereafter, in the local accumulation of PMN. Preceding the influx of leukocytes, the peritoneal fluid became chemotactic for rabbit PMN in vitro. The majority of this activity could be attributed to a cleavage product of C5 (C5a). Similarly, injection of endotoxin into the peritoneal cavity of C5-normal mice resulted in the generation of a chemotactic factor for mouse PMN which was followed by the accumulation of PMN in the peritoneal fluid. In contrast, injection of endotoxin into the peritoneal cavity of C5-deficient mice resulted in the generation of virtually no detectable chemotactic activity and a markedly depressed accumulation of PMN during the first 24 hr after injection. The data suggest that C5 plays an important role in the early phases of PMN accumulation in response to inflammatory stimuli. The rapid accumulation of PMN in response to an inflammatory stimulus such as bacterial endotoxin would be expected to be a major factor in host defense against proliferation and dissemination of infectious agents.
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spelling pubmed-21390222008-04-17 BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES Snyderman, Ralph Phillips, Jean K. Mergenhagen, Stephan E. J Exp Med Article The importance of C5 in the generation of complement (C)-dependent chemotactic activity in vitro is well recognized. However, the actual role C5 may play in the accumulation of polymorphonuclear leukocytes (PMN) at inflammatory sites in vivo has not been established. Injection of glycogen or endotoxin into the peritoneal cavities of guinea pigs resulted, shortly thereafter, in the local accumulation of PMN. Preceding the influx of leukocytes, the peritoneal fluid became chemotactic for rabbit PMN in vitro. The majority of this activity could be attributed to a cleavage product of C5 (C5a). Similarly, injection of endotoxin into the peritoneal cavity of C5-normal mice resulted in the generation of a chemotactic factor for mouse PMN which was followed by the accumulation of PMN in the peritoneal fluid. In contrast, injection of endotoxin into the peritoneal cavity of C5-deficient mice resulted in the generation of virtually no detectable chemotactic activity and a markedly depressed accumulation of PMN during the first 24 hr after injection. The data suggest that C5 plays an important role in the early phases of PMN accumulation in response to inflammatory stimuli. The rapid accumulation of PMN in response to an inflammatory stimulus such as bacterial endotoxin would be expected to be a major factor in host defense against proliferation and dissemination of infectious agents. The Rockefeller University Press 1971-10-31 /pmc/articles/PMC2139022/ /pubmed/5112201 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Snyderman, Ralph
Phillips, Jean K.
Mergenhagen, Stephan E.
BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES
title BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES
title_full BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES
title_fullStr BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES
title_full_unstemmed BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES
title_short BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO : ROLE OF C5 IN THE ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES IN INFLAMMATORY EXUDATES
title_sort biological activity of complement in vivo : role of c5 in the accumulation of polymorphonuclear leukocytes in inflammatory exudates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139022/
https://www.ncbi.nlm.nih.gov/pubmed/5112201
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