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IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
The immunochemical properties of purified A(1) and A(2) glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity d...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139049/ https://www.ncbi.nlm.nih.gov/pubmed/4104425 |
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author | Moreno, Carlos Lundblad, Arne Kabat, Elvin A. |
author_facet | Moreno, Carlos Lundblad, Arne Kabat, Elvin A. |
author_sort | Moreno, Carlos |
collection | PubMed |
description | The immunochemical properties of purified A(1) and A(2) glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity difference. This was also supported by absorption studies: A(2) substance specifically removed antibodies reacting with A(2) substance leaving anti-A(1) activity. A(1) substance was more effective than A(2) substance in dissolving an A(1) anti-A(1)-specific precipitate. Purified γM anti-A hemolyzed A(1) cells more readily than A(2) cells. Inhibition studies using mono- and difucosyl type 2 A-active oligosaccharides showed that type 2 difucosyl receptors are present in A(2) substance. The structural basis for the specificity difference between A(1) and A(2) would appear to be that A(2) substances lack type 1 A determinants; this would account for the observed higher H and Le(b) activity in A(2) substances. |
format | Text |
id | pubmed-2139049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21390492008-04-17 IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A Moreno, Carlos Lundblad, Arne Kabat, Elvin A. J Exp Med Article The immunochemical properties of purified A(1) and A(2) glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity difference. This was also supported by absorption studies: A(2) substance specifically removed antibodies reacting with A(2) substance leaving anti-A(1) activity. A(1) substance was more effective than A(2) substance in dissolving an A(1) anti-A(1)-specific precipitate. Purified γM anti-A hemolyzed A(1) cells more readily than A(2) cells. Inhibition studies using mono- and difucosyl type 2 A-active oligosaccharides showed that type 2 difucosyl receptors are present in A(2) substance. The structural basis for the specificity difference between A(1) and A(2) would appear to be that A(2) substances lack type 1 A determinants; this would account for the observed higher H and Le(b) activity in A(2) substances. The Rockefeller University Press 1971-08-01 /pmc/articles/PMC2139049/ /pubmed/4104425 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Moreno, Carlos Lundblad, Arne Kabat, Elvin A. IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A |
title | IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A |
title_full | IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A |
title_fullStr | IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A |
title_full_unstemmed | IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A |
title_short | IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A |
title_sort | immunochemical studies on blood groups : li. a comparative study of the reaction of a(1)and a(2) blood group glycoproteins with human anti-a |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139049/ https://www.ncbi.nlm.nih.gov/pubmed/4104425 |
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