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IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A

The immunochemical properties of purified A(1) and A(2) glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity d...

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Detalles Bibliográficos
Autores principales: Moreno, Carlos, Lundblad, Arne, Kabat, Elvin A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139049/
https://www.ncbi.nlm.nih.gov/pubmed/4104425
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author Moreno, Carlos
Lundblad, Arne
Kabat, Elvin A.
author_facet Moreno, Carlos
Lundblad, Arne
Kabat, Elvin A.
author_sort Moreno, Carlos
collection PubMed
description The immunochemical properties of purified A(1) and A(2) glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity difference. This was also supported by absorption studies: A(2) substance specifically removed antibodies reacting with A(2) substance leaving anti-A(1) activity. A(1) substance was more effective than A(2) substance in dissolving an A(1) anti-A(1)-specific precipitate. Purified γM anti-A hemolyzed A(1) cells more readily than A(2) cells. Inhibition studies using mono- and difucosyl type 2 A-active oligosaccharides showed that type 2 difucosyl receptors are present in A(2) substance. The structural basis for the specificity difference between A(1) and A(2) would appear to be that A(2) substances lack type 1 A determinants; this would account for the observed higher H and Le(b) activity in A(2) substances.
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spelling pubmed-21390492008-04-17 IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A Moreno, Carlos Lundblad, Arne Kabat, Elvin A. J Exp Med Article The immunochemical properties of purified A(1) and A(2) glycoproteins have been compared to ascertain whether their antigenic determinants differ. Quantitative precipitin and complement-fixation studies using several anti-A sera as well as purified γG anti-A antibodies clearly showed a specificity difference. This was also supported by absorption studies: A(2) substance specifically removed antibodies reacting with A(2) substance leaving anti-A(1) activity. A(1) substance was more effective than A(2) substance in dissolving an A(1) anti-A(1)-specific precipitate. Purified γM anti-A hemolyzed A(1) cells more readily than A(2) cells. Inhibition studies using mono- and difucosyl type 2 A-active oligosaccharides showed that type 2 difucosyl receptors are present in A(2) substance. The structural basis for the specificity difference between A(1) and A(2) would appear to be that A(2) substances lack type 1 A determinants; this would account for the observed higher H and Le(b) activity in A(2) substances. The Rockefeller University Press 1971-08-01 /pmc/articles/PMC2139049/ /pubmed/4104425 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Moreno, Carlos
Lundblad, Arne
Kabat, Elvin A.
IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
title IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
title_full IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
title_fullStr IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
title_full_unstemmed IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
title_short IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LI. A COMPARATIVE STUDY OF THE REACTION OF A(1)AND A(2) BLOOD GROUP GLYCOPROTEINS WITH HUMAN ANTI-A
title_sort immunochemical studies on blood groups : li. a comparative study of the reaction of a(1)and a(2) blood group glycoproteins with human anti-a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139049/
https://www.ncbi.nlm.nih.gov/pubmed/4104425
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