Cargando…

IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE

Preculture treatment of normal spleen cells with antiserum against mouse kappa light chains and complement was found to inhibit in vitro responses of these cells to TNP and erythrocyte (carrier) antigens, primarily by elimination of a thymus-derived helper component required for the response. Spleen...

Descripción completa

Detalles Bibliográficos
Autores principales: Lesley, J. F., Kettman, J. R., Dutton, R. W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139068/
https://www.ncbi.nlm.nih.gov/pubmed/15776565
_version_ 1782143710167826432
author Lesley, J. F.
Kettman, J. R.
Dutton, R. W.
author_facet Lesley, J. F.
Kettman, J. R.
Dutton, R. W.
author_sort Lesley, J. F.
collection PubMed
description Preculture treatment of normal spleen cells with antiserum against mouse kappa light chains and complement was found to inhibit in vitro responses of these cells to TNP and erythrocyte (carrier) antigens, primarily by elimination of a thymus-derived helper component required for the response. Spleen populations inactivated in this way could be reconstituted with irradiated, carrier-immune spleen cells or with carrier-educated thymus-derived spleen cells. The ability of helper populations (i.e. irradiated, carrier-immune spleen cells or carrier-educated thymus-derived spleen cells) to enhance the response of normal spleen cells to hapten was eliminated by pretreatment of the helper cells with anti-kappa serum and complement. No significant effect of anti-kappa and complement treatment on precursor cell populations in normal spleen or bone-marrow-derived spleen could be demonstrated. The data are interpreted as evidence for the presence of immunoglobulin components. The function of these molecules is not established but it would be reasonable to assume that they are involved in antigen recognition, on the surface of thymus-derived cells.
format Text
id pubmed-2139068
institution National Center for Biotechnology Information
language English
publishDate 1971
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21390682008-04-17 IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE Lesley, J. F. Kettman, J. R. Dutton, R. W. J Exp Med Article Preculture treatment of normal spleen cells with antiserum against mouse kappa light chains and complement was found to inhibit in vitro responses of these cells to TNP and erythrocyte (carrier) antigens, primarily by elimination of a thymus-derived helper component required for the response. Spleen populations inactivated in this way could be reconstituted with irradiated, carrier-immune spleen cells or with carrier-educated thymus-derived spleen cells. The ability of helper populations (i.e. irradiated, carrier-immune spleen cells or carrier-educated thymus-derived spleen cells) to enhance the response of normal spleen cells to hapten was eliminated by pretreatment of the helper cells with anti-kappa serum and complement. No significant effect of anti-kappa and complement treatment on precursor cell populations in normal spleen or bone-marrow-derived spleen could be demonstrated. The data are interpreted as evidence for the presence of immunoglobulin components. The function of these molecules is not established but it would be reasonable to assume that they are involved in antigen recognition, on the surface of thymus-derived cells. The Rockefeller University Press 1971-09-01 /pmc/articles/PMC2139068/ /pubmed/15776565 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Lesley, J. F.
Kettman, J. R.
Dutton, R. W.
IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE
title IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE
title_full IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE
title_fullStr IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE
title_full_unstemmed IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE
title_short IMMUNOGLOBULINS ON THE SURFACE OF THYMUS-DERIVED CELLS ENGAGED IN THE INITIATION OF A HUMORAL IMMUNE RESPONSE
title_sort immunoglobulins on the surface of thymus-derived cells engaged in the initiation of a humoral immune response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139068/
https://www.ncbi.nlm.nih.gov/pubmed/15776565
work_keys_str_mv AT lesleyjf immunoglobulinsonthesurfaceofthymusderivedcellsengagedintheinitiationofahumoralimmuneresponse
AT kettmanjr immunoglobulinsonthesurfaceofthymusderivedcellsengagedintheinitiationofahumoralimmuneresponse
AT duttonrw immunoglobulinsonthesurfaceofthymusderivedcellsengagedintheinitiationofahumoralimmuneresponse