Cargando…
REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS
Peritoneal exudate cells (PEC), obtained after the rejection of EL4 leukemia by BALB/c mice, are much more effective in the specific in vitro destruction of (51)Cr-labeled EL4 cells than are spleen, thymus, lymph node, or peripheral blood lymphocytes. The presence of a large number of effector cells...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1972
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139166/ https://www.ncbi.nlm.nih.gov/pubmed/5025438 |
_version_ | 1782143732816019456 |
---|---|
author | Berke, Gideon Sullivan, Karen A. Amos, Bernard |
author_facet | Berke, Gideon Sullivan, Karen A. Amos, Bernard |
author_sort | Berke, Gideon |
collection | PubMed |
description | Peritoneal exudate cells (PEC), obtained after the rejection of EL4 leukemia by BALB/c mice, are much more effective in the specific in vitro destruction of (51)Cr-labeled EL4 cells than are spleen, thymus, lymph node, or peripheral blood lymphocytes. The presence of a large number of effector cells at the site of graft rejection is reflected in the potent cytolytic activity seen in vitro. Effector cells temporarily lose cytolytic reactivity when treated with trypsin but regain reactivity with time. This recovery occurs in normal as well as in immune serum. The destructive reactivity of PEC is increased when macrophages are removed. The remaining population of nonadherent PEC is composed primarily of small- to medium-sized lymphocytes. Complex tissue culture media are not needed, but there is a definite requirement for serum. The required serum component is heat stable, nondialyzable, and is not consumed during the reaction. The use of an ascites allograft system made these observations possible and permitted the isolation of those host cells intimately associated with rejection. |
format | Text |
id | pubmed-2139166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1972 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21391662008-04-17 REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS Berke, Gideon Sullivan, Karen A. Amos, Bernard J Exp Med Article Peritoneal exudate cells (PEC), obtained after the rejection of EL4 leukemia by BALB/c mice, are much more effective in the specific in vitro destruction of (51)Cr-labeled EL4 cells than are spleen, thymus, lymph node, or peripheral blood lymphocytes. The presence of a large number of effector cells at the site of graft rejection is reflected in the potent cytolytic activity seen in vitro. Effector cells temporarily lose cytolytic reactivity when treated with trypsin but regain reactivity with time. This recovery occurs in normal as well as in immune serum. The destructive reactivity of PEC is increased when macrophages are removed. The remaining population of nonadherent PEC is composed primarily of small- to medium-sized lymphocytes. Complex tissue culture media are not needed, but there is a definite requirement for serum. The required serum component is heat stable, nondialyzable, and is not consumed during the reaction. The use of an ascites allograft system made these observations possible and permitted the isolation of those host cells intimately associated with rejection. The Rockefeller University Press 1972-06-01 /pmc/articles/PMC2139166/ /pubmed/5025438 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Berke, Gideon Sullivan, Karen A. Amos, Bernard REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS |
title | REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS |
title_full | REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS |
title_fullStr | REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS |
title_full_unstemmed | REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS |
title_short | REJECTION OF ASCITES TUMOR ALLOGRAFTS : I. ISOLATION, CHARACTERIZATION, AND IN VITRO REACTIVITY OF PERITONEAL LYMPHOID EFFECTOR CELLS FROM BALB/c MICE IMMUNE TO EL4 LEUKOSIS |
title_sort | rejection of ascites tumor allografts : i. isolation, characterization, and in vitro reactivity of peritoneal lymphoid effector cells from balb/c mice immune to el4 leukosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139166/ https://www.ncbi.nlm.nih.gov/pubmed/5025438 |
work_keys_str_mv | AT berkegideon rejectionofascitestumorallograftsiisolationcharacterizationandinvitroreactivityofperitoneallymphoideffectorcellsfrombalbcmiceimmunetoel4leukosis AT sullivankarena rejectionofascitestumorallograftsiisolationcharacterizationandinvitroreactivityofperitoneallymphoideffectorcellsfrombalbcmiceimmunetoel4leukosis AT amosbernard rejectionofascitestumorallograftsiisolationcharacterizationandinvitroreactivityofperitoneallymphoideffectorcellsfrombalbcmiceimmunetoel4leukosis |