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POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)

Effective supernatants (SUP), which potentiate mouse T-cell responses to phytohemagglutin (PHA), are obtained from cells of several species (human, rabbit, rat, mouse) and indeed from syngeneic spleen, thymus, or bone marrow cells. Unstimulated cells release some SUP activity but more is produced af...

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Detalles Bibliográficos
Autores principales: Gery, Igal, Waksman, Byron H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139186/
https://www.ncbi.nlm.nih.gov/pubmed/5033418
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author Gery, Igal
Waksman, Byron H.
author_facet Gery, Igal
Waksman, Byron H.
author_sort Gery, Igal
collection PubMed
description Effective supernatants (SUP), which potentiate mouse T-cell responses to phytohemagglutin (PHA), are obtained from cells of several species (human, rabbit, rat, mouse) and indeed from syngeneic spleen, thymus, or bone marrow cells. Unstimulated cells release some SUP activity but more is produced after stimulation. Lipopolysaccharide (LPS) produced very active SUP in all cultures tested. PHA was similarly active on human leukocytes only, whereas concanavalin A (Con A) gave highly efficient SUP only with mouse spleen cells. SUP production is not correlated with a mitotic response of the donor cells and is observed in cultures unable to respond mitotically to the stimulant. Adherent mouse spleen cell populations, consisting largely or entirely of macrophages, produce active SUP, while nonadherent cells do not. Similarly, purification of human peripheral leukocytes on nylon columns, with removal of macrophages and other adherent cells, destroys their ability to produce SUP. The importance of indirect effects in stimulating mitotic responses of T cells is emphasized by the fact that the mitotic response of mouse thymocytes to LPS and its ability to potentiate the response of these cells to PHA disappears with removal of adherent cells from the thymocyte population. Conversely the production of SUP from spleen cells stimulated by Con A requires the presence of T cells.
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spelling pubmed-21391862008-04-17 POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S) Gery, Igal Waksman, Byron H. J Exp Med Article Effective supernatants (SUP), which potentiate mouse T-cell responses to phytohemagglutin (PHA), are obtained from cells of several species (human, rabbit, rat, mouse) and indeed from syngeneic spleen, thymus, or bone marrow cells. Unstimulated cells release some SUP activity but more is produced after stimulation. Lipopolysaccharide (LPS) produced very active SUP in all cultures tested. PHA was similarly active on human leukocytes only, whereas concanavalin A (Con A) gave highly efficient SUP only with mouse spleen cells. SUP production is not correlated with a mitotic response of the donor cells and is observed in cultures unable to respond mitotically to the stimulant. Adherent mouse spleen cell populations, consisting largely or entirely of macrophages, produce active SUP, while nonadherent cells do not. Similarly, purification of human peripheral leukocytes on nylon columns, with removal of macrophages and other adherent cells, destroys their ability to produce SUP. The importance of indirect effects in stimulating mitotic responses of T cells is emphasized by the fact that the mitotic response of mouse thymocytes to LPS and its ability to potentiate the response of these cells to PHA disappears with removal of adherent cells from the thymocyte population. Conversely the production of SUP from spleen cells stimulated by Con A requires the presence of T cells. The Rockefeller University Press 1972-07-01 /pmc/articles/PMC2139186/ /pubmed/5033418 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gery, Igal
Waksman, Byron H.
POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)
title POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)
title_full POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)
title_fullStr POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)
title_full_unstemmed POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)
title_short POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : II. THE CELLULAR SOURCE OF POTENTIATING MEDIATOR(S)
title_sort potentiation of the t-lymphocyte response to mitogens : ii. the cellular source of potentiating mediator(s)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139186/
https://www.ncbi.nlm.nih.gov/pubmed/5033418
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AT waksmanbyronh potentiationofthetlymphocyteresponsetomitogensiithecellularsourceofpotentiatingmediators