THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE

Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on...

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Detalles Bibliográficos
Autores principales: Andersson, Jan, Melchers, Fritz, Galanos, Chris, Lüderitz, Otto
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139234/
https://www.ncbi.nlm.nih.gov/pubmed/4571328
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author Andersson, Jan
Melchers, Fritz
Galanos, Chris
Lüderitz, Otto
author_facet Andersson, Jan
Melchers, Fritz
Galanos, Chris
Lüderitz, Otto
author_sort Andersson, Jan
collection PubMed
description Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on bone marrow-derived mouse lymphocytes. Within the structure of lipopolysaccharide consisting of lipid A, a core polysaccharide, and the O-polysaccharide antigen, lipid A was found to be the mitogenic part. The mitogenic effect of lipid A, consisting of phosphorylated glucosamine disaccharide units with ester- and amide-linked fatty acids, was lost after alkali treatment, which removes ester-linked fatty acids. Insertion of the lipid A portion of lipopolysaccharides into the lipid bilayer of the plasma membranes of bone marrow-derived lymphocytes is discussed as the initial mitogenic action.
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spelling pubmed-21392342008-04-17 THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE Andersson, Jan Melchers, Fritz Galanos, Chris Lüderitz, Otto J Exp Med Article Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on bone marrow-derived mouse lymphocytes. Within the structure of lipopolysaccharide consisting of lipid A, a core polysaccharide, and the O-polysaccharide antigen, lipid A was found to be the mitogenic part. The mitogenic effect of lipid A, consisting of phosphorylated glucosamine disaccharide units with ester- and amide-linked fatty acids, was lost after alkali treatment, which removes ester-linked fatty acids. Insertion of the lipid A portion of lipopolysaccharides into the lipid bilayer of the plasma membranes of bone marrow-derived lymphocytes is discussed as the initial mitogenic action. The Rockefeller University Press 1973-03-31 /pmc/articles/PMC2139234/ /pubmed/4571328 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Andersson, Jan
Melchers, Fritz
Galanos, Chris
Lüderitz, Otto
THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
title THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
title_full THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
title_fullStr THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
title_full_unstemmed THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
title_short THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES : LIPID A AS THE MITOGENIC PART OF THE MOLECULE
title_sort mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes : lipid a as the mitogenic part of the molecule
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139234/
https://www.ncbi.nlm.nih.gov/pubmed/4571328
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