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IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES

Specific anti-(rabbit cathepsin D) serum, previously shown to inhibit cathepsin D, arrested the intracellular digestion of sheep IgG and radiochemically labeled hemoglobin and proteoglycan in rabbit alveolar macrophages. In the presence of antiserum, cells remained viable, but became very vacuolated...

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Detalles Bibliográficos
Autores principales: Dingle, John T., Poole, A. Robin, Lazarus, Gerald S., Barrett, Alan J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139245/
https://www.ncbi.nlm.nih.gov/pubmed/4267207
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author Dingle, John T.
Poole, A. Robin
Lazarus, Gerald S.
Barrett, Alan J.
author_facet Dingle, John T.
Poole, A. Robin
Lazarus, Gerald S.
Barrett, Alan J.
author_sort Dingle, John T.
collection PubMed
description Specific anti-(rabbit cathepsin D) serum, previously shown to inhibit cathepsin D, arrested the intracellular digestion of sheep IgG and radiochemically labeled hemoglobin and proteoglycan in rabbit alveolar macrophages. In the presence of antiserum, cells remained viable, but became very vacuolated. Both sheep IgG and hemoglobin were demonstrated immunocytochemically in vacuoles most of which could also be shown to contain cathepsin D. When the antiserum was removed, cells regained their normal morphology, and digestion of endocytosed proteins returned to normal. These results indicate that cathepsin D can be inhibited within lysosomes of viable cells, in which it plays a major role in the intracellular digestion of certain proteins.
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spelling pubmed-21392452008-04-17 IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES Dingle, John T. Poole, A. Robin Lazarus, Gerald S. Barrett, Alan J. J Exp Med Article Specific anti-(rabbit cathepsin D) serum, previously shown to inhibit cathepsin D, arrested the intracellular digestion of sheep IgG and radiochemically labeled hemoglobin and proteoglycan in rabbit alveolar macrophages. In the presence of antiserum, cells remained viable, but became very vacuolated. Both sheep IgG and hemoglobin were demonstrated immunocytochemically in vacuoles most of which could also be shown to contain cathepsin D. When the antiserum was removed, cells regained their normal morphology, and digestion of endocytosed proteins returned to normal. These results indicate that cathepsin D can be inhibited within lysosomes of viable cells, in which it plays a major role in the intracellular digestion of certain proteins. The Rockefeller University Press 1973-05-01 /pmc/articles/PMC2139245/ /pubmed/4267207 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Dingle, John T.
Poole, A. Robin
Lazarus, Gerald S.
Barrett, Alan J.
IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
title IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
title_full IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
title_fullStr IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
title_full_unstemmed IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
title_short IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES
title_sort immunoinhibition of intracellular protein digestion in macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139245/
https://www.ncbi.nlm.nih.gov/pubmed/4267207
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