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THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION

An electron microscope study was made of the mode of lymphocyte migration across the endothelial layer of venules in the Peyer's patches of mice and rats. Single and serial sections were examined. Of a total of about 800 lymphocytes observed in single sections, 91% were located between endothel...

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Autor principal: Schoefl, G. I.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139256/
https://www.ncbi.nlm.nih.gov/pubmed/5050723
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author Schoefl, G. I.
author_facet Schoefl, G. I.
author_sort Schoefl, G. I.
collection PubMed
description An electron microscope study was made of the mode of lymphocyte migration across the endothelial layer of venules in the Peyer's patches of mice and rats. Single and serial sections were examined. Of a total of about 800 lymphocytes observed in single sections, 91% were located between endothelial cells and 9% were surrounded by endothelial cytoplasm in the particular plane of section. 62% of the lymphocytes occurred in groups of two or more. In long sequences of serial sections through 21 endothelial cells, all lymphocytes were located external to the endothelial cells though some appeared "internal" at certain levels of sectioning. The probability that a lymphocyte which appears to be surrounded by endothelial cell cytoplasm actually lies within the cell was analyzed with a mathematical model derived from data obtained from single sections. The results of this analysis suggested that at least 93–99% of lymphocytes (within 90% limits of confidence) take an intercellular path in their migration from blood to lymph. It is concluded that lymphocytes migrate across the vascular endothelium by insinuating themselves between endothelial cells and not by passing through them. Rather than constituting an increased barrier to cell migration, the unusual height of the endothelial cells in these vessels is interpreted to be a special adaptation which allows sustained cell traffic without excessive fluid loss taking place concomitantly.
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spelling pubmed-21392562008-04-17 THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION Schoefl, G. I. J Exp Med Article An electron microscope study was made of the mode of lymphocyte migration across the endothelial layer of venules in the Peyer's patches of mice and rats. Single and serial sections were examined. Of a total of about 800 lymphocytes observed in single sections, 91% were located between endothelial cells and 9% were surrounded by endothelial cytoplasm in the particular plane of section. 62% of the lymphocytes occurred in groups of two or more. In long sequences of serial sections through 21 endothelial cells, all lymphocytes were located external to the endothelial cells though some appeared "internal" at certain levels of sectioning. The probability that a lymphocyte which appears to be surrounded by endothelial cell cytoplasm actually lies within the cell was analyzed with a mathematical model derived from data obtained from single sections. The results of this analysis suggested that at least 93–99% of lymphocytes (within 90% limits of confidence) take an intercellular path in their migration from blood to lymph. It is concluded that lymphocytes migrate across the vascular endothelium by insinuating themselves between endothelial cells and not by passing through them. Rather than constituting an increased barrier to cell migration, the unusual height of the endothelial cells in these vessels is interpreted to be a special adaptation which allows sustained cell traffic without excessive fluid loss taking place concomitantly. The Rockefeller University Press 1972-09-01 /pmc/articles/PMC2139256/ /pubmed/5050723 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Schoefl, G. I.
THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION
title THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION
title_full THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION
title_fullStr THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION
title_full_unstemmed THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION
title_short THE MIGRATION OF LYMPHOCYTES ACROSS THE VASCULAR ENDOTHELIUM IN LYMPHOID TISSUE : A REEXAMINATION
title_sort migration of lymphocytes across the vascular endothelium in lymphoid tissue : a reexamination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139256/
https://www.ncbi.nlm.nih.gov/pubmed/5050723
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